Abstract
In anaerobically adapted samples of synchronized cultures of the unicellular green alga Scenedesmus obliquus it was observed that both the rate and the maximum volume of hydrogen produced in the light changed in a parallel fashion over the life cycle. These two parameters of cells of the 16th h were 3 times greater than the comparable values for cells of the 8th h. Although both photosystems are involved in photohydrogen production the patterns seen over a complete life cycle (24 h) for hydrogen metabolism was inverse to that noted for changes in the photosynthetic capacity. The provision of either glucose, ethanol or acetate to 8th and 16th h cultures enhanced photohydrogen production of the 8th to the same level as the 16th h. From these findings, and also from the observation that the starch content is low at the 8th but 4 fold at the 16th h, it is apparent that in autotrophic cultures an endogenous organic compound, and not water, serves as the electron donor for photohydrogen production. Since free glucose was not detected the natural substrate is most likely starch. From experiments with monochromatic light and observations on the inhibitory action of DCMU and DBMIB on photohydrogen production we conclude that the major portion of the machinery for photohydrogen production in Scenedesmus requires both PS I and PS II participation and the input of electrons from the natural substrate proceeds through PS II.
The alternate possibility that glucose, acetate and ethanol also act as inhibitors of reactions, most probably photophosphorylation, which compete with photohydrogen production was suggested by some experiments. The subsequent modulation of hydrogenase activity was discussed as a possible reason for the enhancement of photohydrogen production.
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Abbreviations
- CCCP:
-
carbonylcyanide-m-chlorophenylhydrazone
- Chl:
-
chlorophyll
- DCMU:
-
3-(3′, 4′-dichlorophenyl)-1,1-dimethyl-urea
- DBMIB:
-
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
- NAD:
-
nicotinamide adenine dinucleotide
- PSI:
-
photosystem I
- PSII:
-
photosystem II
- PCV:
-
packed cell volume
References
Banauch, D., Brümmer, W., Ebeling, W., Metz, H., Rindfrey, H., Lang, H., Leybold, K., Rick, W.: Eine Glucose-Dehydrogenase für die Glucose-Bestimmung in Körperflüssigkeiten. Z. Klin. Chem. Klin. Biochem. 13, 101–107 (1975)
Ben-Amotz, A., Gibbs, M.: H2 Metabolism in Photosynthetic Organisms. II. Light-dependent H2 Evolution by Preparations from Chlamydomonas, Scenedesmus and Spinach. Biochem. Biophys. Res. Commun. 64, 355 (1975)
Bishop, N.I.: The photometabolism of glucose by hydrogen adapted algae. Biochim. Biophys. Acta 51, 323–332 (1961)
Bishop, N.I.: Partial reactions of photosynthesis and photoreduction. Ann. Rev. Plant Physiol. 17, 185–208 (1966)
Bishop, N.I., Gaffron, H.: On the interrelation of the mechanisms for oxygen and hydrogen evolution in adapted algae. Reprinted from “Photosynthetic Mechanisms in Green Plants”, Publication 1145, pp. 441–451. National Academy of Sciences-National Research Council 1963
Bishop, N.I., Senger, H.: Preparation and photosynthetic properties of synchronous cultures of Scenedesmus In: Methods in Enzymology, Vol. 23A, pp. 53–66, San Pietro, A., ed. New York: Academic Press 1971
Bishop, N.I., Frick, M., Jones, L.W.: Photohydrogen production in green algae: Water serves as the primary substrate for hydrogen and oxygen production. In: Biological Solar Energy Conversion, pp. 3–22 Mitsui, A. et al., eds. New York: Academic Press 1977
Butler, W.L.: Energy distribution in the photosynthetic apparatus of plants. In: Chlorophyll-Proteins, Reaction Centers and Photosynthetic Membranes. Brookhaven Symp. Biol. 28, 338–346 (1976)
Dean, J.A. Lang Handbook of Chemistry, 11th ed. New York: McGraw Hill 1973
Gaffron, H.: Über auffallende Unterschiede in der Physiologie nahe verwandter Algenstämme, nebst Bemerkungen über “Lichtatmung”. Biol. Zbl. 59 302–313 (1939)
Gaffron, H.: The effect of specific poisons upon the photoreduction with hydrogen in green algae. J. Gen. Physiol. 26, 241–267 (1942)
Gaffron, H.: Photosynthesis, photoreduction and dark reduction of carbon dioxide in certain algae. Biol. Rev. 19, 1–20 (1944)
Gaffron, H., Rubin, J.: Fermentative and photochemical production of hydrogen in algae. J. Gen. Physiol. 26, 219–240 (1942)
Healy, F.P.: The mechanism of hydrogen evolution by Chlamydomonas moewusii. Plant Physiol. 45, 153–159 (1970)
Holden, M.: Chlorophylls. In: Chemistry and Biochemistry of Plant Pigments, pp. 461–488, Goodwin, T.W., ed., London: Academic Press 1965
Jones, L.W., Bishop, N.I.: Simultaneous measurement of oxygen and hydrogen exchange from the blue-green alga Anabaena. Plant Physiol. 57, 659–665 (1976)
Kessler, E.: Hydrogenase, photoreduction and anaerobic growth. In: Algal Physiology and Biochemistry, pp. 456–473, Stewart, W.D.P., ed. Oxford: Blackwell Scientific 1974
Krasna, A.I.: Catalytic and structural properties of the enzyme hydrogenase and its role in biophotolysis of water. In: Biological Solar Energy Conversion, pp. 36–39, Mitsui, A. et al., eds. New York: Academic Press 1977
Loomis, A.G.: In: International Critical Tables, Vol. 3, pp. 255–261, Washburn, E.W., ed. New York: McGraw-Hill 1928
MacKinney, g.: Absorption of light by chlorophyll solution. J. Biol. Chem. 140, 315–322 (1941)
Senger, H.: Charakterisierung einer Synchronkultur von Scenedesmus obliquus, ihrer potentiellen Photosyntheseleistung und des Photosynthese-Quotienten während des Entwicklungszyklus. Planta 90, 243–266 (1970a)
Senger, H.: Quantenausbeute und unterschiedliches Verhalten der beiden Photosysteme des Photosyntheseapparates während des Entwicklungsablaufes von Scenedesmus obliquus in Synchronkulturen. Planta 92, 327–346 (1970b)
Senger, H.: Quantum yield of photosynthesis in synchronous cultures of algae. Proc. Ist Europ. Biophys. Congr., pp. 33–36, Broda, E., Locker, Al., Springer-Lederer, H., eds. Wien: Wiener Medizinische Akademie 1971
Senger, H.: Changes in photosynthetic activities in synchronous cultures of Scenedesmus, Chlorella and Chlamydomonas In: “Les Cycles Cellulaires et leurs Blocages”. Publ. C.N.R.S./Gif 240, 101–108 (1974)
Senger, H., Bishop, N.I.: Changes in the quantum yield and photoreduction during the synchronous life cycle of Scenedesmus obliquus. Nature 214, 140–142 (1967)
Senger, H., Bishop, N.I.: Emerson enhancement effect in synchronous Scenedesmus cultures. Nature 221, 975 (1969a)
Senger, H., Bishop, N.I.: Changes in the photosynthetic apparatus during the synchronous life cycle of Scenedesmus obliquus. In: Progress in Photosynthesis Research, Vol. 1, 425–434, Metzner, H., ed. Tübingen 1969b
Senger, H., Frickel-Faulstich, B.: The regulation of electron flow in synchronized cultures of green algae. 3rd Intern. Congr. on Photosynthesis, Rehovot 1974, pp. 715–727, Avron, M., ed. Amsterdam: Elsevier 1974
Senger, H., Pfau, J., Werthmüller, K.: Continuous cultivation of homocontinuous and synchronized microalgae. In: Methods in Cell Physiology, Vol. V, pp. 301–323, Prescott, D.M., ed. New York: Academic Press 1972
Spruit, C.J.P.: Simultaneous photoproduction of hydrogen and oxygen by Chlorella. Meded. Landbouwhogesch. Wageningen 58, 1–17 (1958)
Stuart, T.S., Gaffron, H.: The mechanism of hydrogen photoproduction by several algae. 1. The effect of inhibitors of photophosphorylation. Planta 106, 91–100 (1972a)
Stuart, T.S., Gaffron, H.: The gas exchange of hydrogen-adapted algae as followed by mass spectrometry. Plant Physiol. 50, 136–140 (1972b)
Tanner, W., Loos, E., Klob, W., Kandler, O.: The quantum requirement for light dependent anaerobic glucose assimilation by Chlorella vulgaris. Z. Pflanzenphysiol. 59, 301–303 (1968)
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Senger, H., Bishop, N.I. Observations on the photohydrogen producing activity during the synchronous cell cycle of Scenedesmus obliquus . Planta 145, 53–62 (1979). https://doi.org/10.1007/BF00379927
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DOI: https://doi.org/10.1007/BF00379927