Oecologia

, Volume 65, Issue 4, pp 487–491 | Cite as

Differential response of Daphnia genotypes to oxygen stress: respiration rates, hemoglobin content and low-oxygen tolerance

  • Lawrence J. Weider
  • Winfried Lampert
Original Papers

Summary

Laboratory respiration rate experiments using three electrophoretically identified clones of the fresh water, planktonic cladoceran, Daphnia pulex, from an eutrophic farm pond, indicated that clones acclimated to both low and high oxygen levels, regulated oxygen consumption across a wide range of oxygen concentrations (1.0–9.0 mg· liter-1). A “threshold” oxygen level of 0.5–1.0 mg·liter-1 was reached, where animals succumbed to oxygen stress, regardless of hemoglobin content. No significant clonal differences in respiration rates were found. These data suggest that members of this Daphnia population are able to regulate oxygen metabolism across a wide range of ambient oxygen concentrations, and indicate a well-adapted respiratory system.

Low-oxygen tolerance experiments and hemoglobin measurements indicated further that physiological differences indeed exist between clones; one clone produced the lowest amount of hemoglobin and was least tolerant of low oxygen levels. These data imply that spatial and temporal changes in dissolved oxygen concentration may be an important selective force influencing the clonal (genotypic) composition of natural cladoceran populations.

Keywords

Oxygen Level Hemoglobin Content Oxygen Stress Farm Pond Daphnia Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Belman BW, Childress JJ (1976) Circulatory adaptations to the oxygen minimum layer in the bathypelagic mysid Gnathophausia ingens. Biol Bull 150:15–37Google Scholar
  2. Carvalho GR (1984) Haemoglobin synthesis in Daphnia magna Straus (Crustacea: Cladocera): ecological differentiation between neighboring populations. Freshwat Biol 14:501–506Google Scholar
  3. Chandler A (1954) Causes of variation in the hemoglobin content of Daphnia (Crustacea: Cladocera) in nature. Proc Zool Soc Lond 124:625–630Google Scholar
  4. Childress JJ (1968) Oxygen minimum layer, vertical distribution and respiration of the mysid Gnathophausia ingens. Science 160:1242–1243Google Scholar
  5. Childress JJ (1971) Respiratory adaptations to the oxygen minimum layer in the bathypelagic mysid Gnathophausia ingens. Biol Bull 141:109–121Google Scholar
  6. Childress JJ (1975) The respiratory rates of midwater crustaceans as a function of depth of occurrence and in relation to the oxygen minimum off southern California. Comp Biochem Physiol 50:787–799Google Scholar
  7. Childress JJ (1976) Physiological approaches to the biology of midwater crustaceans. In: Anderson N (ed) Predictions of Sonic Scattering Layer, Plenum, New York, pp 37–80Google Scholar
  8. Davis JC (1975) Minimal dissolved oxygen requirements of aquatic life with emphasis on Canadian species: a review. J Fish Res Bd Can 32:2295–2332Google Scholar
  9. Fox HM (1945) The oxygen affinities of certain invertebrate hemoglobins. J Exp Biol 21:161–165Google Scholar
  10. Fox HM (1948) The haemoglobin of Daphnia. Proc R Soc Lond B Biol Sci 135:195–212Google Scholar
  11. Fox HM (1954) Oxygen and haem in invertebrates. Nature 174:355Google Scholar
  12. Green J (1956) Variation in the haemoglobin content of Daphnia. Proc R Soc Lond B Biol Sci 145:214–232Google Scholar
  13. Hebert PDN, Crease TJ (1980) Clonal coexistence in Daphnia pulex (Leydig): Another planktonic paradox. Science 207:1363–1365Google Scholar
  14. Heisey D, Porter K (1977) The filtering and respiration rates of Daphnia magna and Daphnia galeata. LImnol Oceanogr 22:839–845Google Scholar
  15. Herbert MR (1954) The tolerance of oxygen deficiency in the water by certain cladocera. Mem Ist Ital Idrobiol 8:97–107Google Scholar
  16. Hutchinson GE (1967) A Treatise on Limnology, Vol 2. Wiley, New YorkGoogle Scholar
  17. Kobayashi M (1981) The haemoglobin concentration in relation to the body size in Daphnia magna. Sci Rep Niigata Univ Ser D (Biol) 18:15–19Google Scholar
  18. Kobayashi M (1982a) Influence of body size on haemoglobin concentration and resistance to oxygen deficiency in Daphnia magna. Comp Biochem Physiol 72A:599–602Google Scholar
  19. Kobayashi M (1982b) Relationship between oxygen consumption and resistance to oxygen deficiency of Daphnia magna. Comp Biochem Physiol 73A:239–241Google Scholar
  20. Kobayashi M, Hoshi T (1984) Analysis of respiratory role of haemoglobin in Daphnia magna. Zool Science 1:523–532Google Scholar
  21. Lampert W (1984) The measurement of respiration. In: Downing JA, Rigler FH (eds) A manual on methods for the assessment of secondary productivity in fresh waters, 2nd ed, Blackwell, Oxford, pp 413–468Google Scholar
  22. Landon MS, Stasiak RH (1983) Daphnia haemoglobin concentration as a function of depth and oxygen availability in Arco Lake, Minnesota. Limnol Oceanogr 28:731–737Google Scholar
  23. Lehninger AL (1975) Biochemistry, 2nd ed, Worth, New YorkGoogle Scholar
  24. Lynch M (1983) Ecological genetics of Daphnia. Evolution 37:358–374Google Scholar
  25. Lynch M, Weider LJ, Lampert W (1985) Measuring the carbon balance in Daphnia. Limnol Oceanogr (in press)Google Scholar
  26. Obreshkove V, Banta AM (1930) A study of the rate of oxygen consumption in different Cladocera clones derived from the same mother. Physiol Zool 3:1–8Google Scholar
  27. Ray AA (1982) SAS User's Guide, Statistics 1982. SAS Inst Cary, North CarolinaGoogle Scholar
  28. Sokal RR, Rohlf FJ (1981) Biometry, 2nd ed, WH Freeman and Co., San FranciscoGoogle Scholar
  29. Terwilliger RC (1980) Structures of invertebrate hemoglobins. Amer Zool 20:53–67Google Scholar
  30. Vos J, Bernaerts F, Gabriels I, Decleir W (1979) Aerobic and anaerobic respiration of adult Artemia salina L., acclimated to different oxygen concentrations. Comp Biochem Physiol 62A:545–548Google Scholar
  31. Weber RE (1980) Functions of invertebrate hemoglobins with special reference to adaptations to environmental hypoxia. Amer Zool 20:79–101Google Scholar
  32. Weider LJ (1984a) Spatial heterogeneity of Daphnia genotypes: Vertical migration and habitat partitioning. Limnol Oceanogr 29:225–235Google Scholar
  33. Weider LJ (1984b) Spatial and Temporal Genetic Heterogeneity in a Natural Daphnia Population: Ecological and Physiological Differences Between Genotypes, unpubl Ph D dissertation, Univ of Illinois, Urbana-ChampaignGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Lawrence J. Weider
    • 1
  • Winfried Lampert
    • 1
  1. 1.Arbeitsgruppe PlanktonökologieMax-Planck-Institut für LimnologiePlönFederal Republic of Germany

Personalised recommendations