Abstract
The biological transformation from rifamycin B to rifamycin S was carried out with the live whole cells of Humicola sp., ATCC 20620, immobilized in a dual hollow fiber bioreactor (DHFBR). Humicola sp., inoculated in the DHFBR, proliferated successfully to a high density cell mass within the space between an outer silicone tubing and three inner polypropylene hollow fiber membranes. In order to control the cell growth a nitrogen deficient medium was fed. Conversion of rifamycin B continued for more than 30 d, whereas that of immobilized rifamycin B oxidase lasted only for 3 d in comparable conditions.
In the DHFBR the volumetric productivity of rifamycin S was 0.65–1.03 mmol/(dm3 · h) with 60% conversion, while that in the rotating packed disk reactor was 0.27 mmol/(dm3 · h) with 40% conversion at a residence time of 0.5–1.5 h.
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Lancini, G.; Zanichelli, W.: Structure-activity relationship among the semisynthetic antibiotics. In: D. Perman (Ed.), pp. 521–546. London: Academic Press 1977
Han, M. H.; Seong, B. L.; Son, H. J.; Mheen, T. I.: Rifamycin B oxidase from Monocillium sp., a new type of diphenol oxidase. FEBS Lett. 151 (1983) 36–40
Seong, B. L.; Son, H. J.; Mheen, T. I.; Park, Y. H.; Han, M. H.: Enzymatic oxidation of rifamycins by a microorganism of the genus Humicola. J. Ferment. Technol. 63 (1985) 515–522
Seong, B. L.; Han, M. H.: A facile preparation of rifamycin derivates by use of manganese dioxide. Chemistry Lett. 5 (1982) 627–628
Kim, E. K.; Choi, C. Y.; Park, J. M.; Han, M. H.; Park, Y. H.: Effects of pH and glucose concentration on production of rifamycin oxidase J. Ferment. Technol. 62 (1984) 117–121
Lee, G. M.; Choi, C. Y.: The properties of immobilized whole cell of Humicola sp. with rifamycin oxidase activity. Biotechnol. Lett. 6 (1984) 143–148
Chung, B. H.; Chang, H. N.; Han, M. H.: Immobilization and characterization of rifamycin B oxidase in cellulose acetate beads. Kor. J. Appl. Microbiol. Bioeng. 13 (1985) 115–118
Lee, G. M.; Choi, C. Y.; Park, J. M.; Han, M. H.: Biotransformation of rifamycin B to rifamycin S using immobilised whole cells of Humicola sp. in a fluidised bed reactor. J. Chem. Tech. Biotechnol. 35B (1984) 3–10
Chung, B. H.; Chang, H. N.; Han, M. H.: Enzymatic conversion of rifamycin B in a rotating packed disk reactor. J. Ferment. Technol. 6 (1986) 343–345
Chang, H. N.; Chung, B. H.; Kim, I. H.: Dual hollow-fiber bioreactor for aerobic whole cell immobilization. Am. Chem. Soc. Symp. Ser. 314 (1986) 32–42
Chung, B. H.; Chang, H. N.; Kim, I. H.: High density cell cultivation of E. coli in a dual hollow fiber bioreactor. Kor. J. Appl. Microbiol. Bioeng. 13 (1985) 209–212
Chung, B. H.; Chang, H. N.; Kim, I. H.: Rifamycin B production by Nocardia mediterranei immobilized in a dual hollow fiber bioreactor. Enzyme Microb. Technol. 9 (1987) 345–349
Hwang, J. S.; Chang, H. N.: Continuous production of acrylamide by Brevibacterium sp. immobilized in a dual hollow fiber bioreactor. Biotechnol. Lett. 9 (1987) 237–242
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Hwang, Y.B., Chung, B.H., Chang, H.N. et al. Biological conversion of rifamycin B by live Humicola sp. cells immobilized in a dual hollow fiber bioreactor. Bioprocess Engineering 3, 159–163 (1988). https://doi.org/10.1007/BF00379651
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DOI: https://doi.org/10.1007/BF00379651