Summary
Artifactual formation of N-nitrosodimethylamine during the extraction procedure from aminopyrine and nitrite was examined. The use of the basic pH condition was the most effective in preventing artifactual formation. Sulfamic acid or ascorbic acid was partially effective in preventing artifactual formation. Since significant losses of volatile N-nitrosodimethylamine occur during the extraction and concentration steps, we analyzed N-nitrosodimethylamine by combined gas chromatography mass spectrometry with 15N-nitrosodimethylamine as an internal standard. The use of a fused silica capillary column enabled us to obtain a fine separation of the chromatogram. This methodology was applied to our model experiment, which was performed to locate the formation of N-nitrosodimethylamine when a rabbit was exposed to NO2 after the administration of aminopyrine. SO3 inhaled together with NO2 was found to increase the nitrosation.
Similar content being viewed by others
References
Angeles RM, Keefer LK, Roller PP, Uhm SJ (I978) Chemical models for possible nitrosamine artifact formation in environmental analysis. In: Walker EA, Griciute L, Castegnaro M, Lyle RE (eds) Environmental aspect of N-nitroso compounds. IARC Sci Pub [No 19] pp 109–115
Challis BC (1981) The chemistry of formation of N-nitroso compounds. In: Gibson GG, Ioannides C (eds) Safety evaluation of nitrosatable drugs and chemicals. Taylor & Francis, Ltd, London, pp 16–55
Fine DH (1980) N-nitroso compounds in the environment. In: Pitts JJN, Metcalf RL (eds) Advances in environ. science and technol, vol 10. John Wiley & Sons, New York, pp 39–123
Garland WA, Holowaschenko H, Kuenzig W, Norkus EP, Conney AH (1982) A high resolution mass spectrometry assay for N-nitrosodimethylamine in human plasma. In: Magee PN (ed) Nitrosamines and human cancer. BanBury Report [No 12] pp 183–196
Iqbal ZM, Dahl K, Epstein SS (1980) Role of nitrogen dioxide in the biosynthesis of nitrosamines in mice. Science 207:1475–1477
Kamm JJ, Dashman T, Conney AH, Burns JH (1974) The effect of ascorbate on amine-nitrite hepatotoxicity. In: Bogovsky P, Walker EA (eds) N-nitroso compounds in the environment. IARC Sci Pub [No 9] pp 200–204
Kawabata T, Ohshima H (1979) Studies on the method for analysis on N-nitroso compounds. Mutagens Toxicol [Jpn] 7:104–114
Kusumoto S, Kimura T, Nakajima T, Nakamura A (1981) Formation of nitrosodimethylamine by NO2 exposure in rats pretreated with aminopyrine. In: Takemura N, Yamamura Y (eds) Proc 8th Int Conf Occup Health Chem Ind. Aikawa Shobo Publishing Co, Tokyo, pp 48–55
Mirvish SS, Karlowski K, Sams JP, Arnold SD (1978) Studies related to nitrosamide formation. In: Walker EA, Griciute L, Castegnaro M, Lyle RE (eds) Environmental aspect of N-nitroso compounds. IARC Sci Pub [No 19] pp 161–174
Rounbehler DP, Ross R, Fine DH, Iqbal ZM, Epstein SS (1979) Quantitation of dimethylnitrosamine in the whole mouse after biosynthesis in vivo from trace level of precursors. Science 197:917–918
Sander J, Schweinsberg FU, Menz HP (1968) Untersuchungen über die Entstehung cancerogener Nitrosamine im Magen. Hoppe Seylers Z Physiol Chem 349:1691–1697
Uozumi M, Kusumoto S, Kimura T, Nakamura A, Nakajima T (1982) Formation of N-nitrosodimethylamine by NOx exposure in rats and rabbit pretreated with aminopyrine. In: Bartsch H, O'Neill IK, Castegnaro M, Okada M (eds) N-nitroso compounds: occurrence and biological effects. IARC Sci Pub [No 41] pp 425–432
Author information
Authors and Affiliations
Additional information
Supported by a grant in aid for environmental science from the Ministry of Education, Science and Culture of Japan
Rights and permissions
About this article
Cite this article
Kosaka, H., Uozumi, M. & Nakajima, T. Measurement of nitrosodimethylamine by capillary gas chromatography-mass spectrometry with the 15N-labelled compound as an internal standard. Int. Arch Occup Environ Heath 54, 233–239 (1984). https://doi.org/10.1007/BF00379052
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00379052