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Application of rat mast cell incubates as a possible short-time test for sensitizing occupational chemicals

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Summary

The direct action of sensitizing occupational chemicals (formaldehyde, phenol, phenylhydrazine, p-aminophenol) on rat mast cells was investigated by determination of histamine using HPLC separation and fluorimetric detection. It turned out that dispensed mast cells from immunized and non-immunized Wistar-rats are more sensitive than small-cut lung tissue slices.

Passive cutaneous anaphylaxis was negative after a fortnight sensitizing experiment with the here described occupational chemicals. Short-time tests with rat mast cells reflect anaphylactoid response and are suitable for the screening of sensitizing chemicals.

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Authors and Affiliations

  1. Institut für Arbeitsphysiologie an der Universität Dortmund, Ardeystr. 67, D-4600, Dortmund 1, Federal Republic of Germany

    F. Diel & B. Neidhart

  2. Institut für Laboratoriumsmedizin, Brauhausstr. 4, D-4600 Dortmund 1, Federal Republic of Germany

    W. Oprée

Authors
  1. F. Diel
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  2. B. Neidhart
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  3. W. Oprée
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Additional information

Part of this study was supported by a grant of the Deutsche Forschungsgemeinschaft (Di 239/1-4)

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Diel, F., Neidhart, B. & Oprée, W. Application of rat mast cell incubates as a possible short-time test for sensitizing occupational chemicals. Int. Arch Occup Environ Heath 48, 369–373 (1981). https://doi.org/10.1007/BF00378685

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  • DOI: https://doi.org/10.1007/BF00378685

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