Summary
We have isolated five mutants of Candida maltos, which lack the 2-aminoadipate reductase activity, an enzyme involved in the lysine biosynthesis. By means of complementation analysis using protoplast fusion, the isolated mutants were divided into two complementation groups. Thereof the C. maltosa strain G457 could be transformed by the plasmids pDP12 and pDP13, which contain the L YS2-coding gene of Saccharomyces cerevisiae. On the basis of our presented results obtained by studies on hybridization, stability, and recovery of plasmids from C. maltosa transformants, we suggest that transformation does proceed integratively.
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Abbreviations
- AA :
-
2-aminoadipate
- PRA :
-
phosphoribosylanthranilate
References
Bhattacharjee JK (1985) CRC Crit Rev Microbiol 12:131–151
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1523
Bode R, Melo C, Birnbaum D (1985) Biochem Physiol Pflanzen 180:301–308
Böttcher F, Samsonova IA (1978) Z Allg Mikrobiol 18:637–646
Botstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Gene 8:17–24
Bulat SA, Peshekhonov VT, Chepurnaya OV, Zakharov IA (1985) Curr Genet 9:119–121
Case MA (1983) Transformation in fungi. In: Lurquin PIT, Leinhofs A (eds) Genetic engineering in eukaryotes. Plenum, pp 1–5
Chang MC, Jung HK, Suzuki T, Takagi M, Yano K (1984) J Gen Appl Microbiol 30:489–498
Chevallier MR, Aigle M (1979) FEBS Lett 108:179–180
Chainault AC, Carbon J (1979) Gene 5:111–126
Cregg JM, Barringer KJ, Hessler AY, Madden KR (1985) Mol Cell Biol 5:3376–3385
Davidow LS, Apostlakos D, O'Donnell MM, Proctor AR, Orgrydziak DM, Wing RA, Stasko I, DeZeeuw JR (1985) Curr Genet 10:39–48
Eibel H, Philippsen P (1984) Nature 307:386–388
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Gaillardin C, Ribet AM, Heslot H (1985) Curr Genet 10:49–55
Hanahan D (1983) J Mol Biol 166:557–580
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:153–158
Kingsman AJ, Clarke L, Mortimer RK, Carbon J (1979) Gene 7:141–152
Klinner U, Bottcher F (1984) Z AlIg Mikrobiol 24:539–544
Klinner U, Samsonova IA, Bottcher F (1984) Curr Microbiol 11:241–246
Kunze G, Hecker M, Birnbaum D (1984) Z Allg Mikrobiol 24:33–40
Kunze G, Petzoldt C, Bode R, Samsonova I, Hecker M, Birnbaum D (1985) Curr Genet 9:205–209
Kurtz MB, Cotelyou MW, Kirsch DR (1986) Mol Cell Biol 6:142–149
Livingston DM, Kupfer DM (1977) J Mol Biol 116:249–260
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275
Panzeri L, Philippsen P (1982) EMBO J 1:1605–1611
Roggenkamp R, Hansen H, Eckart M, Janowicz Z, Hollenberg CP (1986) Mol Gen Genet 202:302–308
Schmidt H, Bode R, Lindner M, Birnbaum D (1985) J Basic Microbiol 25:675–681
Simchen G, Winston F, Styles CA, Fink GR (1984) Proc Natl Acad Sci USA 81:2431–2434
Sinha AK, Bhattacharjee JK (1970) Biochem Biophys Res Commun 39:1205–1210
Southern EM (1975) J Mol Biol 98:503–517
Storts DR, Bhattacharjee JK (1984) Annu Meet Am Soc Microbiol H119
Tully M, Gilbert HJ (1985) Gene 36:235–240
Wright APH, Maundrell K, Shall S (1986) Curr Genet 10:503–508
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Kunze, G., Bode, R., Schmidt, H. et al. Identification of a lys2 mutant of Candida maltosa by means of transformation. Curr Genet 11, 385–391 (1987). https://doi.org/10.1007/BF00378181
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DOI: https://doi.org/10.1007/BF00378181