Abstract
Clonal assays for haematopoietic progenitors were performed on mononuclear cells isolated from bone marrow samples collected from the sternebrae of normal horses. Colony-forming units-erythroid (CFUE), burst-forming units-erythroid (BFU-E), colony-forming units-granulocyte/monocyte (CFU-GM) and colony-forming units-fibroblastic (CFU-F) were assayed, and reference values for clinically normal horses were established. The mean numbers of CFU-E, BFU-E and CFU-GM per 5 × 104 cells were 329 ± 48, 30 ± 5 and 131 ± 13, respective. The mean number of CFU-F was 49 ± 6 per 2 ± 10 cells. The numbers of CFU-E and BFU-E were linearly related to the concentrations of fetal bovine serum (FBS), bovine serum albumin (BSA) and the cell number plated. The number of CFU-E were increased in the presence of erythropoietin (EPO) but its presence was not required for colony growth. BFU-E had an absolute requirement for EPO but the number of BFU-E was not linearly related to dose of EPO. Methods for clonal assays of equine hematopoietic progenitors are described.
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Swardson, C.J., Kociba, G.J. Clonogenic haematopoietic progenitor assays from clinically normal horses. Comparative Haematology International 6, 187–193 (1996). https://doi.org/10.1007/BF00378109
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DOI: https://doi.org/10.1007/BF00378109