Abstract
Analysis of available nucleotide sequence data for class I HLA genes has established that the seventh intron is one of the gene regions which expresses the highest degree of locus specificity (the percentage sequence divergence between nonallelic genes minus the percentage sequence divergence between allelic genes). We have subcloned short DNA sequences including this region from the HLA-Cw3 gene. Two clones, pC250 and pC800, were tested by hybridizing them at high stringency to a panel of clones containing class I HLA genes. Under conditions permitting a strong hybridization signal with a C-locus gene, pC800 also expressed a weak but significant hybridization to other class I genes, while pC250 appeared to hybridize exclusively to the C-locus gene. Hybridization of the pC250 probe at high stringency to Hind III-digested genomic DNA from a panel of unrelated individuals and homozygous typing cell lines revealed a single band in all cases. However, equivalent hybridization against Eco RI-digested DNA revealed two hybridization bands, one at 7.9 kb which correlated with the serologically defined Cw5 and Cw8 alleles, and one at 7.6 kb which correlated with the Cw1, Cw2, Cw3, Cw4, Cw6, and Cw7 alleles.
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Baur, M. P., Neugebauer, M., Deppe, H., Sigmund, M., Luton, T., Mayr, W. R., and Albert, E. D.: Population analysis on the basis of deduced haplotypes from random families. In E. D. Albert, M.P. Baur, and W.R. Mayr (eds.): Histocompatibility Testing 1984, pp. 333–341, Springer-Verlag, Berlin, 1984
Biro, P. A., Pan, J., Sood, A. K., Kole, R., Reddy, V. B., and Weissman, S. M.: Sequences of human repetitive DNA, nonglobulin genes and major histocompatibility locus genes. III. The major histocompatibility complex. Cold Spring Harbor Symp. Quant. Biol. 47: 1079–1086, 1983
Cann, H. M., Ascanio, L., Paul, P., Marcadet, A., and Dausset, J.: Polymorphic restriction endonuclease fragment segregates and correlates with the gene for HLA-B8. Proc. Natl. Acad. Sci. U.S.A. 80: 1665–1668, 1983
Cohen, D., Paul, P., Font, M. P., Cohen, O., Sayagh, B., Marcadet, A., Busson, M., Mahouy, G., Cann, H. M., and Dausset, J.: Analysis of HLA class I genes with restriction endonuclease fragments. Implications for polymorphisms of the human major histocompatibility complex. Proc. Natl. Acad. Sci. U.S.A. 80: 6289–6292, 1983
Coppin, H. L., Weissman, S. M., and McDevitt, H. O.: A study of HLA-B-locus polymorphism with a specific hybridization probe. In E. D. Albert, M. P. Baur, and W. R. Mayr (eds.): Histocompatibility Testing 1984, pp. 554–556, Springer-Verlag, Berlin, 1984
Driesel, A. J., Römer, K., Schunter, F., Laryea, M. D., Schneider, E. M., Wernet, P., Henke, J., Basler, M., and Kömpf, J.: DNA polymorphism of the major histocompatibility class I genes and their association with serologically defined haplotypes. Immunogenetics 21: 529–538, 1985
Erlich, H. A., Stettler, M., Sheng-Dong, R., Ness, D., and Grumet, F. C.: Segregation and mapping analysis of polymorphic HLA class I restriction fragments: Detection of a novel fragment. Science 222: 72–74, 1983
Feinberg, A. P. and Vogelstein, B.: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6–13, 1983
Grumet, F. C., Fish, L., Moossazedeh, J., Ness, D., and Duceman, B.: HLA class I gene typing with an HLA-B-specific DNA probe. In E. D. Albert, M. P. Baur, and W. R. Mayr (eds.): Histocompatibility Testing 1984, pp. 551–554, Springer-Verlag, Berlin, 1984
Jordan, B. R., Caillol, D., Damotte, M., Delovitch, T., Ferrier, P., Kahn-Perles, B., Kourilsky, F., Layet, C., Le Bouteiller, P., Lemonnier, F. A., Malissen, M., N'Guyen, C., Sire, J., Sodoyer, R., Strachan, T., and Trucy, J.: HLA class I genes: From structure to expression, serology and function. Immunol. Rev. 84: 73–92, 1985
Koller, B. H. and Orr, H. T.: Cloning and complete sequence of an HLA-A2 gene: Analysis of two HLA-A alleles at the nucleotide level. J. Immunol. 134: 2727–2733, 1985
Koller, B. H., Sidwell, B., DeMars, R., and Orr, H. T.: Isolation of HLA-locus specific DNA probes from the 3′-untranslated region. Proc. Natl. Acad. Sci. U.S.A. 81: 5175–5178, 1984
Kunkel, L. M., Smith, K. D., Boyer, S. H., Borgaonkar, D. S., Watchel, S. S., Miller, O. J., Breg, W. R., Jones Jr., H. W., and Rary, J. M.: Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc. Natl. Acad. Sci. U.S.A. 74:1245–1249, 1977
Malissen, M., Malissen, B., and Jordan, B. R.: Exon/intron organization and complete nucleotide sequence of an HLA gene. Proc. Natl. Acad. Sci. U.S.A. 79: 893–897, 1982
N'Guyen, C., Sodoyer, R., Trucy, J., Strachan, T., and Jordan, B. R.: The HLA-AW24 gene: Sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes. Immunogenetics 21: 479–489, 1985
Sodoyer, R., Damotte, M., Delovitch, T. L., Trucy, J., Jordan, B. R., and Strachan, T.: Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-Cw3). EMBO J. 3: 879–885, 1984
Sodoyer, R., N'Guyen, C., Strachan, T., Santoni, M. J., Damotte, M., Trucy, J., and Jordan, B. R.: Allelism in the HLA class I multigene family. Ann. Inst. Pasteur 136 C: 71–84, 1985
Southern, E. M.: Detection of specific sequences among DNAfragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517, 1975
Strachan, T., Sodoyer, R., Damotte, M., and Jordan; B. R.: Complete nucleotide sequence of a functional class I HLA gene, HLA-A3. Implications for the evolution of HLA genes. EMBO J. 3: 887–894, 1984
Ways, J. P. and Parham, P.: The complete primary structure of HLA-Bw58. J. Biol. Chem., in press, 1985
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Strachan, T., Dodge, A.B., Smillie, D. et al. An HLA-C-specific DNA probe. Immunogenetics 23, 115–120 (1986). https://doi.org/10.1007/BF00377971
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DOI: https://doi.org/10.1007/BF00377971