Abstract
We have already developed nine B10.MOL congenic strains carrying H-2 haplotypes derived from Japanese wild mice, Mus musculus molossinus, with the C57BL/10 genetic background. To obtain monoclonal antibodies against the H-2 antigen of the Japanese wild mouse, we carried out cell fusion using spleen cells from the animal immunized with one of the B10.MOL strains, B10.MOL-SGR (H-2 wm7). As a result, 19 hybridomas producing monoclonal antibodies were produced. Analysis with the intro-H-2 recombinants derived from B10.MOL-SGR indicated that 8 of them reacted with the class I and II with the class II molecule. The class I antibodies were tested for their cross -reactivities on wild mice and on the panels of standard inbred and B10.MOL strains. Most of the antibodies reacted with both the Japanese wild mice and the other subspecies, including standard inbred, while two antibodies highly specific for the donor H-2K region reacted with only three wild-derived mice, two M. m. molossinus from Anj o and Shizuoka, Japan, and one M. m. domesticus from Pigeon, Canada. In addition, all of the other four antibodies reactive with the K antigen of B10.MOL-SGR also reacted with the same three wild mice. The wild mice belonging to different subspecies might share very similar H-2K antigenic determinants in spite of their genetic and geographical remoteness.
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References
Arden, B. and Klein, J.: Biochemical comparison of major histocompatibility complex molecules from different subspecies of Mus musculus: Evidence for trans-specific evolution of alleles. Proc. Natl. Acad. Sci. U.S.A. 79: 2342–2346, 1982
Arden, B., Wakeland, E. K., and Klein, J.: Structural comparisons of serologically indistinguishable H-2K-encoded antigens from inbred and wild mice. J. Immunol. 125: 2424–2428, 1980
Arden, B., Wakeland, E. K., and Klein, J.: Minor structural variants of H-2K-controlled molecules in wild mice. Immunogenetics 16: 491–493, 1982
Duncan, W. R. and Klein, J.: Histocompatibility-2 system in wild mice. IX. Serological analysis of 13 new B10.W congenic lines. Immunogenetics 10: 45–65, 1980
Figueroa, E., Davis, W. C., and Klein, J.: Ten new monoclonal antibodies detecting antigenic determinants on class I H-2 molecules. Immunogenetics 14: 177–180, 1981
Figueroa, F., Klein, D., Tewarson, S., and Klein, J.: Evidence for placing the Neu-1 locus within the mouse H-2 complex. J. Immunol. 129: 2089–2093, 1982a
Figueroa, F., Tewarson, S., Neufeld, E., and Klein, J.: H-2 haplotypes of strains DBR7, B10.NZW, NFS, BQ2, STU, TO1, and TO2. Immunogenetics 15: 431–436, 1982b
Hämmerling, G. J., Hammerling, U., and Lemke, H.: Isolation of twelve monoclonal antibodies against Ia and H-2 antigens. Serological characterization and reactivity with B and T lymphocytes. Immunogenetics 8: 433–445, 1979
Hauptfeld, V., Nahm, M., Hauptfeld, M., and Shreffler, D. C.: Monoclonal antibodies to mouse MHC antigens. I. Serologic characterization of ten anti-H-2 and anti-Ia reagents. Immunogenetics 19: 169–173, 1984
Klein, J.: Histocompatibility-2 system in wild mice. I. Identification of five new H-2 chromosomes. Transplantation 13: 291–299, 1972
Klein, J.: Biology of the Mouse Histocompatibility-2 Complex, Springer-Verlag, New York, Heidelberg, Berlin, 1975
Klein, J. and Figueroa, F.: Polymorphism of the mouse H-2 loci. Immunol. Rev. 60: 23–57, 1981
Klein, J., Huang, H. S., Lemke, H., Hämmerling, G. J., and Hämmerling, U.: Serological analysis of H-2 and la molecules with monoclonal antibodies. Immunogenetics 8: 419–432, 1979
Klein, J., Götze, D., Nadeau, J. H., and Wakeland, E. K.: Population immunogenetics of murine H-2 and t system. In R. J. Berry (ed.): Biology of the House Mouse, pp. 439–453, Academic Press, London, 1981
Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497, 1975
Lemke, H., Hämmerling, G. J., Höhmanns, O., and Rajewsky, K.: Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249–251, 1978
Moriwaki, K., Shiroishi, T., Minezawa, M., Aotsuka, T., and Kondo, K.: Frequency distribution of histocompatibility-2 antigenic specificities in the Japanese wild mice genetically remote from the European subspecies. J. Immunogenet. 6: 99–113, 1979
Moriwaki, K., Shiroishi, T., and Sagai, T.: H-2 antigenic specificities in B10.MOL H-2 congenic mouse strains. Ann. Rep. Natl. Inst. Genet. (Japan) 30: 34–37, 1980
Moriwaki, K., Miyashita, N., and Yonekawa, H.: Genetic survey of the origin of laboratory mice and its implication in genetic monitoring. In H. C. Rowsell (ed.): Proceedings of ICLAS-CALAS International Symposium on Laboratory Animal Science in 1983, pp. 237–247, Gustav Fisher Verlag, Stuttgart, 1985
Ozato, K. and Sachs, D. H.: Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317–321, 1981
Ozato, K., Mayer, N., and Sachs, D. H.: Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533–540, 1980
Shiroishi, T., Sagai, T., and Moriwaki, K.: A simplified micro-method for cytotoxicity testing using a flat-type titration plate for the detection of H-2 antigens. Microbial. Immunol. 25: 1327–1334, 1981
Shiroishi, T., Sagai, T., and Moriwaki, K.: A new wild derived-H-2 haplotype enhancing K-IA recombination. Nature 300: 370–372, 1982
Tewarson, S., Figueroa, F., and Klein, J.: Identification of D-locus alleles in mouse B10.W lines. Immunogenetics 16: 273–278, 1982a
Tewarson, S., Figueroa, F., and Klein, J.: Monoclonal antibodies specific for class I and class II molecules controlled by the mouse H-2 complex. Immunogenetics 16: 373–379, 1982b
Vollmers, H. P., Eulitz, M., and Götze, D.: Reactivity of hybridoma antibodies specific for H-2 antigens with cells of inbred and wild mice. Immunogenetics 8: 447–459, 1979
Yonekawa, H., Moriwaki, K., Gotoh, O., Hayashi, J., Watanabe, J., Miyashita, N., Pertras, M. L., and Tagashira, Y.: Evolutionary relationships among five subspecies of Mus musculus based on restriction enzyme cleavage patterns of mitochondrial DNA. Genetics 98: 801–816, 1981
Zaleska-Rutczynska, Z. and Klein, J.: Histocompatibility-2 system in wild mice. V. Serologic analysis of sixteen B10.W congenic lines. J. Immunol. 119: 1903–1911, 1977
Zaleska-Rutczynska, Z., Figueroa, F., and Klein, J.: Sixteen new H-2 haplotypes derived from wild mice. Immunogenetics 18: 189–203, 1983
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Sagai, T., Shiroishi, T., Moriwaki, K. et al. Characterization of newly isolated monoclonal antibodies against MHC of a Japanese wild mouse. Immunogenetics 24, 361–367 (1986). https://doi.org/10.1007/BF00377953
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DOI: https://doi.org/10.1007/BF00377953