Summary
The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive temperature and vice versa. The results showed that the primary cold-sensitive defect in crib-1 is in the function, rather than the synthesis, of a molecule needed for the production of the 17S rRNA component of the 37S subunit. Pulse-labeling experiments showed that at 10 °C crib-1 synthesizes the 2.4-Mdal pre-rRNA molecule which contains the sequences for the 17S, 5.8S and 25S rRNA species, but that processing of this molecule is defective such that relatively few stable 17S rRNA molecules accumulate.
Similar content being viewed by others
References
Attardi G, Amaldi R (1970) Prog Nucleic Acid Res Mol Biol 6:219–257
Brown DD (1967) Curr Top Dev Biol 2:47–80
Burdon RH (1971) Prog Nucleic Acid Res Mol Biol 11:33–79
Maden BEH (1976) Trends Biochem Sciences 1:196–199
Perry RP (1976) Prog Nucleic Acid Res Mol Biol 6:219–257
Perry RP (1976) Annu Rev Biochem 45:605–629
Russell PJ, Wilkerson WM (1980) Exp Mycol 4:281–337
Russell PJ, Hammett JR, Selker EU (1976a) J Bacteriol 125:1112–1119
Russell PJ, Hammett JR, Selker EU (1976b) J Bacteriol 127:785–793
Schlitt SC, Russell PJ (1974) J Bacteriol 120:666–671
Warner JR (1974) In: Nomura M, Tissieres A, Lengyel P (eds) Ribosomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 461–488
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Russell, P.J., Selker, E.U. & Jackson, J.A. Cold-sensitive mutation in Neurospora crassa affecting the production of 17S ribosomal RNA from ribosomal precursor RNA. Curr Genet 4, 1–5 (1981). https://doi.org/10.1007/BF00376778
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00376778