Summary
Immunohistochemical and immunochemical analyses were performed on a monoclonal antibody designated 1-2B7B which was derived from immunizing mice with human prostate epithelial tissue. The 1-2B7B antigen was expressed not only along the acinous basement membrane zone (BMZ) of the prostate and testis, but also along the BMZ of the epithelia of several other organs including the skin, oesophagus, urinary bladder, ureter, stomach, intestine and bile duct. The antigenic epitope was not expressed in these tissues of lower mammals. Immunoelectron microscopic studies on normal human skin revealed that the 1-2B7B antigen was localized mainly just beneath the hemidesmosomes of basal keratinocytes, but not beneath melanocytes. Indirect immunofluorescence and immunoelectron microscopic studies on 1 M NaCl-split skin confirmed that this antigen was not separated from the cytoplasmic membrane of basal cells after salt treatment. SDS polyacrylamide gel electrophoresis of immunochemically purified protein from the epidermis demonstrated the molecular weight of the antigen to be 120 kDa. 1-2B7B monoclonal antibody should be a useful probe for studying the pathomechanism of some blistering diseases, as well as the assembly and function of the epidermal-dermal junction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Caughman SW, Krieg T, Timpl R, Hintner H, Katz SI (1987) Nidogen and heparan sulfate proteoglycan: detection of newly isolated basement membrane components in normal and epidermolysis bullosa skin. J Invest Dermatol 89:547–550
Fine J-D, Couchman JR (1988) Chondroitin-6-sulfate-containing proteoglycans: a new component of human skin dermoepidermal junction. J Invest Dermatol 90:283–288
Fine J-D, Horiguchi Y, Couchman JR (1989) 19-DEJ-1, a hemidesmosome-anchoring filament complex-associated monoclonal antibody: definition of a new skin basement membrane antigenic defect in junctional and dystrophic epidermolysis bullosa. Arch Dermatol 125:520–523
Fine J-D, Horiguchi Y, Jester J, Couchman JR (1989) Detection and partial characterization of a midlamina lucida — hemidesmosome-associated antigen (19-DEJ-1) present within human skin. J Invest Dermatol 92:825–830
Foidart JM, Bere EW Jr, Yaar M, Rennard SI, Gullino M, Martin GR, Katz SI (1980) Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein. Lab Invest 42:336–342
Horiguchi Y, Imamura S (1986) Discrepancy between the localization of in vivo bound immunoglobulins in the skin and in vitro binding sites of circulating anti-BMZ antibodies in bullous pemphigoid: immunoelectron microscopic studies. J Invest Dermatol 87:715–719
Horiguchi Y, Abrahamson D, Fine J-D (1991) Epitope mapping of the laminin molecule in murine skin basement membrane zone: demonstration of spatial differences in ultrastructural localization. J Invest Dermatol 96:309–313
Horiguchi Y, Couchman JR, Ljubimov AV, Yamasaki H, Fine J-D (1989) Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in human skin and other basement membranes. J Histochem Cytochem 37:961–970
Horiguchi Y, Fine J-D, Ljubimov AV, Yamasaki H, Couchman JR (1989) Entactin: ultrastructural localization of an ubiquitous basement membrane glycoprotein in mouse skin. Arch Dermatol Res 281:427–432
Jones JCR (1988) Characterization of a 125 K glycoprotein associated with bovine epithelial desmosomes. J Cell Sci 89:207–216
Jones JCR, Goldman RD (1985) Intermediate filaments and the initiation of desmosome assembly. J Cell Biol 101:506–517
Jordon RE, Kawana S, Fritz KA (1985) Immunopathologic mechanism in pemphigus and bullous pemphigoid. J Invest Dermatol 85 [Suppl]:72s-78s
Klatte DH, Kurpakus MA, Grelling KA, Jones JCR (1989) Immunochemical characterization of three components of the hemidesmosome and their expression in cultured epithelial cells. J Cell Biol 109:3377–3390
Labib RS, Anhalt GJ, Patel HP, Mutasim DF, Diaz LA (1986) Molecular heterogeneity of the bullous pemphigoid antigens as detected by immunoblotting. J Immunol 136:1231–1235
Laurie GW, Leblond CP, Martin GR (1982) Localization of type IV collagen, laminin, heparan sulfate proteoglycan and fibronectin to the basal lamina of basement membranes. J Cell Biol 95:340–344
Miller K, Mattey D, Measures H, Hopkins C, Garrod D (1987) Localization of the protein and glycoprotein components of bovine nasal epithelial desmosomes by immunoelectron microscopy. EMBO J 6:885–889
Mutasim DF, Takahashi Y, Labib RS, Anhalt GJ, Patel HP, Diaz LA (1985) A pool of pemphigoid antigen(s) is intracellular and associated with the basal cell cytoskeleton — hemidesmosome complex. J Invest Dermatol 84:47–53
Naito K, Morioka S, Ogawa H (1982) The pathogenic mechanisms of blister formation in bullous pemphigoid. J Invest Dermatol 79:303–306
Pehamberger H, Gschnait F, Konrad K, Holubar K (1980) Bullous pemphigoid, herpes gestanionis and linear dermatitis herpetiformis: circulating anti-basement membrane zone antibodies; in vitro studies. J Invest Dermatol 74:105–108
Sakai LY, Keene DR, Morris NP, Burgeson RE (1986) Type VII collagen is a major structural component of anchoring fibrils. J Cell Biol 103:1577–1586
Schmelz M, Duden R, Cowin P, Franke WW (1986) A constitutive transmembrane glycoprotein of Mr. 165000 (desmoglein) in epidermal and non-epidermal desmosomes. I. Biochemical identification of the polypeptide. Eur J Cell Biol 42:177–183
Schmelz M, Duden R, Cowin P, Franke WW (1986) A constitutive transmembrane glycoprotein of Mr. 165000 (desmoglein) in epidermal and non-epidermal desmosomes. II. Immunolocalization and microinjection studies. Eur J Cell Biol 42:184–199
Shimizu H, McDonald JN, Kennedy AR, Eady RAJ (1989) Demonstration of intra- and extracellular localization of bullous pemphigoid antigen using cryofixation and freeze substitution for postembedding immunoelectron microscopy. Arch Dermatol Res 281:443–448
Sugi T, Hashimoto T, Hibi T, Nishikawa T (1989) Production of human monoclonal anti-basement membrane zone (BMZ) antibodies from a patient with bullous pemphigoid (BP) by Epstein-Barr virus transformation: analyses of the heterogeneity of anti-BMZ antibodies in BP sera using them. J Clin Invest 84:1050–1055
Tanaka T, Korman NJ, Shimizu H, Eady RAJ, Klaus-Kovtun V, Cehrs K, Stanley JR (1990) Production of rabbit antibodies against carboxy-terminal epitopes encoded by bullous pemphigoid cDNA. J Invest Dermatol 94:617–623
Tanaka T, Parry ADD, Klaus-Kovtun V, Steinert PM, Stanley JR (1991) Comparison of molecularly cloned bullous pemphigoid antigen to desmoplakin I confirms that they define a new family of cell adhesion junction plague protein. J Biol Chem (in press)
Tsujimura K, Park YH, Miyama-Inaba M, Meguro T, Ohno T, Ueda M, Masuda T (1990) Comparative studies on FcR functions of murine B cells. J Immunol 144:4571–4578
Westgate GE, Weaver AC, Couchman JR (1985) Bullous pemphigoid antigen localization suggests an intracellular association with hemidesmosomes. J Invest Dermatol 84:218–224
Yamasaki Y, Nishikawa T (1983) Ultrastructural localization of in vitro binding sites of circulating anti-basement membrane antibodies in bullous pemphigoid. Acta Derm Venereol (Stockh) 63:501–506
Yaoita H, Foidart J-M, Katz S (1978) Localization of the collagenous component in skin basement membrane. J Invest Dermatol 70:191–193
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhang, X.M., Horiguchi, Y., Ueda, M. et al. 1-2B7B: Monoclonal antibody reacting to the 120 kDa polypeptide component of human epidermal hemidesmosomes. Arch Dermatol Res 283, 310–316 (1991). https://doi.org/10.1007/BF00376619
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00376619