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Changes in the density of nuclear estrogen receptors in urogenital tissue during pseudopregnancy in the rabbit

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Abstract

To elucidate the possible role of progesterone in pregnancy-induced urinary incontinence, the concentration of nuclear estrogen receptors (E2R) was measured in the urogenital tissues of non-pregnant (controls) and on days 6, 12 and 18 of pseudopregnancy in the rabbit. Whereas in the uterus E2R levels decreased by at least five-fold by day 6 of pseudopregnancy, changed little through day 12 and increased again at day 18, in the vagina and urethra only a small and insignificant decrease and increase occurred at days 6–12 and day 18, respectively. The receptor affinity, as judged by the computed K D values, did not change significantly in any of the preparation during pseudopregnancy. Similar changes in the E2R density were observed in rabbits treated with progesterone. These data provide further and concrete evidence for the tissue specificity in the downregulation of E2R by progesterone.

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References

  1. Iosif S, Batra S, Ek A, Åstedt B. Estrogen receptors in the human female lower urinary tract. Am J Obstet Gynecol 1981;141:718–820

    Google Scholar 

  2. Stanton SL, Kerr-Wilson R, Harris G. The incidence of urological symptoms in normal pregnancy. Br J Obstet Gynaecol 1980;87:897–891

    PubMed  Google Scholar 

  3. Batra S, Iosif S. Progesterone receptors in the female lower urinary tract. J Urol 1987;138:1301–1304

    PubMed  Google Scholar 

  4. Batra S, Iosif CS. Female urethra: a target for estrogen action. J Urol 1983;129:418–420

    PubMed  Google Scholar 

  5. Batra S, Bjellin L, Iosif S, Mårtensson L, Sjögren C. Effect of oestrogen and progesterone on the blood flow in the lower urinary tract of the rabbit. Acta Physiol Scand 1985;123:191–194

    PubMed  Google Scholar 

  6. Batra S, Bjellin L, Sjögren C, Iosif S, Widmark E. Increases in blood flow of the female rabbit urethra following low-dose estrogens. J Urol 1986;136:1360–1362

    PubMed  Google Scholar 

  7. Batra S, Iosif CS. Tissue-specific effects of progesterone on progesterone and estrogen receptors in the female urogenital tract. J Steroid Biochem 1989;32:35–39

    Article  PubMed  Google Scholar 

  8. Batra S, Owman C, Sjöberg N-O, Thorbert G. Relationship between plasma and uterine oestradiol in pseudopregnant rabbits. J Reprod Fertil 1979;56:1–5

    PubMed  Google Scholar 

  9. Thorbert G, Batra S, Owman C, Rosengren E, Sjöberg N-O. Uterine norepinephrine levels as related to plasma and tissue progesterone in pseudopregnant rabbits. Endocrinology 1976;99:1207–1212

    PubMed  Google Scholar 

  10. Laudanski T, Batra S, Åkerlund M. Prostaglandin-induced luteolysis in pregnant and pseudopregnant rabbits and the resultant effects on myometrial activity. J Reprod Fertil 1979;56:141–148

    PubMed  Google Scholar 

  11. Batra S. Subcellular distribution and cytosolic receptors of progesteron and estradiol 17-β in the rabbit myometrium: effect of progesterone treatment. Biol Reprod 1979;21:483–489

    PubMed  Google Scholar 

  12. Batra S, Iosif CS. Effect of estrogen treatment on the peroxidase activity and estrogen receptors in the female rabbit urogenital tissues. J Urol 1992;148:935–938

    PubMed  Google Scholar 

  13. Peterson GL. A simplification of the protein assay method of Lowry et al., which is more generally acceptable. Anal Biochem 1979;83:346–356

    Google Scholar 

  14. Takeda A, Leavitt WW. Progesterone-induced down-regulation of nuclear estrogen receptor in uterine decidual cells. Analysis of receptor synthesis and turnover by the density-shift method. Biochem Biophys Res Commun 1986;135:98–106

    PubMed  Google Scholar 

  15. Batra S. Hormonal control of myometrial function. In: Grudzinskas JG, Yovich JL, Simpson J, Chard T (eds) Cambridge reviews in human reproduction Cambridge, Cambridge University Press (in press)

  16. Batra S. Effect of estrogen and progesterone treatment on calcium uptake by the myometrium and smooth muscle of the lower urinary tract. Eur J Pharmacol 1986;127:37–42

    Article  PubMed  Google Scholar 

  17. Iosif S. Stress incontinence during pregnancy and in puerperium. Int J Gynecol Obstet 1981;19:13–20

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University Hospital, S-221 85, Lund, Sweden

    C. S. Iosif & S. Batra

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  1. C. S. Iosif
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  2. S. Batra
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Iosif, C.S., Batra, S. Changes in the density of nuclear estrogen receptors in urogenital tissue during pseudopregnancy in the rabbit. Int Urogynecol J 5, 264–268 (1994). https://doi.org/10.1007/BF00376236

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