Summary
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1)
We have identified by electron microscopy and isolated a circular DNA species of approximately 6 µm contour length from DNase treated mitochondrial fractions of the petite negative yeast Schizosaccharomyces pombe (S. pombe).
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2)
Another molecular species of about 3 µm length is also present in mitochondrial fractions. These molecules, however, disappear after DNase treatment or extensive washing, indicating their extramitochondrial location. There is evidence (Fournier et al. 1981) that these molecules represent a multicopy plasmid coding for the genes of cytoplasmic ribosomal RNAs.
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3)
A restriction enzyme cleavage map of the 6 µm species was constructed using twelve enzymes. Physical mapping revealed a genome length of approximately 18.9 kilobase pairs, thus confirming the electron microscopic data.
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4)
Northern hybridization of mitochondrial RNA with restriction fragments of mitochondrial DNA revealed two major signals which are attributed to the small and large ribosomal RNA. Apparently both rRNA genes map close together.
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5)
Spontaneous mit− deletion mutants were characterized phenotypically and the location of their deletions was determined.
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6)
In strains carrying a cytoplasmic mutator (Seitz-Mayr and Wolf 1982) or derived from mutator strains four restriction sites have been mapped, which are not present in the mitochondrial DNA of the parental strain. These extra sites are very likely consequences of the action of the mutator.
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7)
In conclusion we have presented evidence that the 6 µm circular DNA species is the mitochondrial genome of fission yeast. Special features of this genome are discussed in comparison with other mitochondrial genomes.
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References
Alwine JC, Kemp PJ, Stark GP (1977) Proc Natl Acad Sci USA 74:5350–5354
Anderson S, Bankier AT, Barell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger G, Schreier PH, Smith AJH, Staden R, Yound IG (1981) Nature (London)290:457–465
Bernardi G, Prunell A, Fonty G, Kopecka H, Strauss I (1976) In: Saccone C, Kroon AM (eds) The Genetic Function of Mitochondrial DNA. North Holland, Amsterdam, pp 259–272
Bibb MJ, Van Etten RA, Wright CT, Walberg MH, Clayton DA (1981) Cell 26:167–180
Borst P, Flavell RA (1976) In: Fasman GD (ed) Handbook of Biochemistry and Molecular Biology, Vol 2, 3rd edn. CRC Press, Cleveland, Ohio, pp 363–374
Borst P, Grivell LA (1981) Nature (London) 290:443–444
Bostock CJ (1969) Biochim Biophys Acta 195:579–581
Clark-Walker GD, Azad AA (1980) Nucleic Acids Res 8:1009–1022
Clark-Walker GD, McArthur CR, Daley DJ (1981) Curr Genet 4:7–12
Del Giudice L, Wolf K, Sassone-Corsi P, Alvino C (1978) Mol Gen Genet 164:289–293
Egel R, Kohli J, Thuriaux P, Wolf K (1980) Annu Rev Genet 14:77–108
Fournier P, Gaillardin C, de Louvencourt L, Heslot H, Lang B, Kaudewitz F (1981) Yeast Newsletters 30:80
Goldbach PW, Borst P, Bollen-de Boer JE, Van Bruggen EFJ (1978) Biochim Biophys Acta 521:169–186
Groot GSP, Van Harten-Loosbroek N (1980) Curr Genet 1:133–135
Kleinschmidt AK (1968) In: Kaplan NO, Colowick SP (eds) Methods in enzymology, Vol XII B. Academic Press, New York, pp 361–377
Lang B, Burger G, Wolf K, Bandlow W, Kaudewitz F (1975) Mol Gen Genet 137:353–363
Larinov VL, Grishin AV, Smirnov MN (1980) Gene 12:41–49
Leupold U (1950) CR Lab Carlsberg Ser Physiol 24:381–480
Macino G, Scazzocchio C, Waring RB, McPhail Berks M, Wayne Davis R (1980) Nature (London) 288:404–406
Manna F, Del Giudice L, Schreil WH, Wolf K (1982) Curr Genet 5:187–189
McMaster GK, Carmichael GG (1977) Proc Natl Acad Sci USA 74:4835–4838
Montoya J, Ojala D, Attardi G (1981) Nature (London) 290:465–470
Morimoto R, Lewin A, Rabinowitz M (1979) Mol Gen Genet 170:1–9
Müller W, Gauthier F (1975) Eur J Biochem 54:385–394
Needleman RB, Tzagoloff A (1975) Anal Biochem 1:545–550
O'Connor RM, McArthur CP, Clark-Walker GD (1975) Eur J Biochem 53:137–144
Ojala D, Montoya J, Attardi G (1981) Nature (London) 290:470–474
Perlman PS, Alexander NJ, Hanson DK, Mahler H (1980) In: Dean DH, Johnson LF, Kimball PD, Perlman PS (eds) Gene structure and expression. Ohio State University Press, Columbus, pp 211–253
Prunell A, Bernardi G (1977) J Mol Biol 110:53–74
Rigby PW, Dieckman M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Sanger F, Coulson AR, Friedman T, Air GM, Barell BG, Brown NL, Fiddes IC, Hutchison III CA, Slocombe PM, Smith MJ (1978) J Mol Biol 125:225–246
Seitz-Mayr G, Wolf K (1982) Proc Natl Acad Sci USA 79:2618–2622
Sriprakash KS, Clark-Walker GD (1980) Biochem Biophys Res Commun 93:186–193
Szybalski W (1976) In: Fasman GD (ed) CRC Handbook of biochemistry and molecular biology. CRC Press, Cleveland
Tabak HF, Weijers PJ (1976) FEBS Lett 69:211–215
Wesolowski M, Algeri A, Fukuhara H (1981) Curr Genet 3:157–162
Wolf K, Burger G, Lang B, Kaudewitz F (1976) Mol Gen Genet 144:67–81
Wolf K, Lang B, Del Giudice L, Anziano P, Perlman PS (1982) In: Mitochondrial Genes. Cold Spring Harb Symp. Cold Spring Harbour Laboratory, New York, pp 335–360
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Anziano, P.Q., Perlman, P.S., Lang, B.F. et al. The mitochondrial genome of the fission yeast schizosaccharomyces pombe . Curr Genet 7, 273–284 (1983). https://doi.org/10.1007/BF00376072
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DOI: https://doi.org/10.1007/BF00376072