Summary
The pattern of aldehyde oxidase (AO) activity was determined in wing discs of Drosophila melanogaster larvae homozygous for the mutants apt 73n, Beaded, and vestigial (vg) in order to determine if reduction in field size in the pouch could be related to alterations of the wild-type AO pattern, as suggested by the Kauffman (1978) hypothesis. The pattern in wild-type discs was resolved into six areas for comparison with mutant discs. vg discs developed at 25° C showed restriction of the pattern into a small area on the anterior side of the disc, and comparison of vg and wild-type prepupal wings allowed positive identification of the AO pattern elements which remained. AO patterns in vg wing discs grown at 27°, 29°, and 31° C were progressively more complete and similar to wild-type, reflecting the reduction in cell death in discs grown at higher temperatures. These results show that cell loss during the third instar in vg development at 25° C is responsible for the alteration of the AO pattern, rather than field size reduction, and that determination of the pattern must take place much earlier than the time of its first appearance during the third larval instar, and before cell death in vg discs begins. Thus mutants acting at earlier stages will be necessary for further tests of the Kauffman hypothesis.
Similar content being viewed by others
References
Altorfer N (1977) apt73n: apterous 73n. DIS 52:2
Bownes M, Roberts S (1981) Regulative Properties of Wing Discs from the Vestigial Mutant of Drosophila melanogaster. Differentiation 18:89–96
Bunow B, Kernevez JP, Joly G, Thomas D (1980) Pattern Formation by Reaction-Diffusion distribution: Application to Morphogenesis in Drosophila. J Theoret Biol 84:629–649
Bryant PJ (1974) Determination and Pattern Formation in the Imaginai Discs of Drosophila. Curr Topics Dev Biol 8:41–80
Bryant PJ (1975) Pattern Formation in the Imaginal Wing Disc of Drosophila melanogaster: Fate Map, Regeneration and Duplication. J Exp Zool 193:49–78
Bryant PJ (1978) Pattern Formation in Imaginal Discs. In: Ashburner M, Wright TRF, (eds) The genetics and biology of Drosophila, vol. 2C:230–325. Academic Press, New York
Dale L, Bownes M (1980) Is Regeneration in Drosophila the Result of Epimorphic Regulation? Wilhelm Roux's Arch 189:91–96
Dickinson WJ (1970) The genetics of Aldehyde Oxidase in Drosophila melanogaster. Genetic 66:487–496
Dickinson WJ (1971) Aldehyde Oxidase in Drosophila melanogaster. A system for genetic studies on genetic regulation. Dev Biol 26:77–86
Fristrom D (1968) Cellular Degeneration in Wing Development of the Mutant Vestigial of Drosophila melanogaster. J Cell Biol 39:488–491
Fristrom D (1969) Cellular Degeneration in the Production of Some Mutant Phenotypes in Drosophila melanogaster MGG 103:363–379
Janning W (1972) Aldehyde Oxidase as a Cell Marker for Internal Organs in Drosophila melanogster. Naturwissenschaften 59:516–517
Janning W (1973) Distribution of Aldehyde Oxidase Activity in Imaginal Disks of Drosophila melanogaster. Dros Inform Sev 50:151
Kauffman S, Shymka RM, Trabert K (1978) Control of Sequential Compartment Formation in Drosophila. Science 199:259–270
Kuhn DT, Cunningham GN (1976) Aldehyde Oxidase Activity in the Tumorous-Head Strain of Drosophila melanogaster. Dev Biol 52:43–51
Kuhn DT, Cunningham GN (1977a) Aldehyde Oxidase Compartmentalization in Drosophila melanogaster Wing Imaginal Disks. Science 196:875–877
Kuhn DT, Cunningham GN (1977b) Aldehyde Oxidase Distribution in Haltere Disks of Homoeotic Bithorax Mutants in Drosohpila melanogaster. Mol Gen Genet 150:37–42
Kuhn DT, Zust B, Illmensee K (1979) Autonomous Differentiation of the Tumorous-head Phenotype in Drosophila melanogaster. Mol Gen Genet 168:117–124
Kuhn DT, Fogerty SC, Eskens AAC, Sprey TE (1983) Developmental Compartments in the Drosophila melanogaster Wing Disc. Dev Biol 95:399–413
Lacalli TC, Harrison LG (1979) Turing's Conditions and the Analysis of Morphogenetic Models. J Theor Biol 76:419–436
Li J, Tsui Y (1936) The Development of Vestigial Wings Under High Temperature in Drosophila melanogaster. Genetics 21:248–263
Lindsley DL, Grell EH (1967) Genetic Variations of Drosophila melanogaster. Carnegie Inst Wash Publication # 627
McCrady E, Smith DL (1984) Comparison of Wing Margin Structures of vestigial and Wild-type Drosophila melanogaster grown at 31° C. Experientia 40:1387–1388
Meinhardt H (1983) Cell determination boundaries as organizing regions for secondary embryonic fields. Dev Biol 96:375–385
Milner MJ, Bleasby AJ, Kelly SL (1984) The Role of the Peripodial Membrane of leg and wing imaginal discs of Drosophila melanogaster during evagination and differentiation in vitro. Wilhelm Roux's Arch 193:180–186
O'Brochta D, Bryant P (1983) Cell Degeneration and Elimination in the Imaginal Wing Disc, Caused by the Mutations Vestigial and Ultravestigial of Drosophila melanogaster. Wilhelm Roux's Arch 192:285–294
O'Brochta D, Bryant P (1985) A Zone of Non-Proliferating Cells at a Lineage Restriction Boundary in Drosophila. Nature 313:138–141
Papageorgiou S, Venieratos D (1983) A Reaction-Diffusion Theory of Morphogenesis with Inherent Pattern Invariance under Scale Variations. J Theor Biol 100:57–97
Russell MA (1985) Positional Information in Insect Segments. Dev Biol 108:269–283
Sprey TE (1977) Aldehyde Oxidase Distribution in the Imaginal Discs of Some Diptera. Wilhelm Roux's Arch 183:1–15
Sprey TE (1971) Cell Death During the Development of the Imaginal Disks of Calliphora erythrocephala. Neth J Zool 21:221–264
Sprey TE, Oldenhave M (1974) A Detailed Organ Map of the Wing Disk of Calliphora erythrocephala. Neth J Zool 24:291–310
Sprey TE, Eskens AAC, Kuhn DT (1982) Enzyme Distribution Patterns in the Imaginal Wing Disc of Drosophila melanogaster and other Diptera. Wilhelm Roux's Arch 191:301–308
Sprey TE, Segal D, Sprey-Pieters HE, Kuhn DT (1981) Influence of Homoeotic Genes on the Aldehyde Oxidase Pattern in Imaginal Discs of Drosophila Melanogaster. Dev Genet 2:75–87
Stanley WF (1931) The Effect of Temperature on Vestigial Wing in Drosophila malanogaster with Temperature-Effective Periods. Physiol Zool 4:394–408
Waddington CH (1940) The Control of Wing Develoment in Drosophila. J Genet 41:75–137
Whittle R, Sprey TE (1982) Correlation between Adult Transformation and Aldehyde Oxidase Staining Pattern in Wing Discs of Apterous Genotypes in Drosophila melanogaster. Wilhelm Roux's Arch 191:285–288
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McCrady, E., Sprey, T.E. The determination of aldehyde oxidase activity patterns in the wing discs of Drosophila melanogaster . Roux's Arch Dev Biol 195, 338–343 (1986). https://doi.org/10.1007/BF00376067
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00376067