Summary
The stage-specific appearance of calliphorin in cuticles of Calliphora vicina was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The fate of the protein, injected into last instar larvae, was pursued by autoradiography of histological sections. Fractionation of sclerotized pupal cuticle in buffer-soluble, urea-soluble and NaOH-soluble fractions shows that calliphorin forms covalent and non-covalent links with other cuticle components. Calliphorin traverses the epidermal cells and enters the cuticle in an undegraded state and appears to be an important constituent of the sclerotizing system.
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König, M., Agrawal, O.P., Schenkel, H. et al. Incorporation of calliphorin into the cuticle of the developing blowfly, Calliphora vicina . Roux's Arch Dev Biol 195, 296–301 (1986). https://doi.org/10.1007/BF00376062
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DOI: https://doi.org/10.1007/BF00376062