Summary
A muscarinic cholinergic receptor is present on undifferentiated cells of the chick embryo. Stimulation of the muscarinic receptor with muscarinic agonists triggers intracellular Ca2#x002B; mobilization. Here, we investigate the effect of phorbol 12-myristate 13-acetate (PMA) on the muscarinic receptor-mediated Ca2#x002B; mobilization, which is monitored in cell suspensions of chick embryos of stage 24 by chlorotetracycline fluorescence. PMA inhibits the Ca2#x002B; mobilization in a time-dependent and concentration-dependent manner without changing the ED50 of acetylcholine. The concentration of PMA that gives halfmaximal inhibition is 3.1×10−9 M PMA.
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Schmidt, H., Oettling, G. & Drews, U. Inhibition of muscarinic receptor-mediated Ca27#x002B; mobilization by phorbol 12-myristate 13-acetate in chick embryo cells. Roux's Arch Dev Biol 197, 37–39 (1988). https://doi.org/10.1007/BF00376039
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DOI: https://doi.org/10.1007/BF00376039