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Measurement of S phase duration in human epidermis using cyclin immunostaining and 3H-thymidine pulse labelling

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Summary

Cyclin/PCNA (proliferating cell nuclear antigen) is a nuclear protein strongly associated with DNA replication sites. Under methanol fixation, cyclin immunostaining might serve as a specific marker for S-phase cells. Associated with single labelling with 3H-thymidine, this immunodetection represents an easy method for measuring S-phase duration. Its application to six samples of normal human epidermis allowed us to measure an S phase of 9.7±0.3 h, a result in fair agreement with a previous estimate of 10.2 h obtained from a classical doublelabelling technique with two different concentrations of 3H-thymidine.

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Authors and Affiliations

  1. Department of Dermatology, Hospital Erasme, Free University of Brussels, B-1070, Brussels, Belgium

    E. Berlingin & M. Heenen

  2. Laboratory of Cytology and Experimental Cancerology and Biology Unit, Institute for Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium

    P. Galand

Authors
  1. E. Berlingin
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  2. M. Heenen
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  3. P. Galand
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Berlingin, E., Heenen, M. & Galand, P. Measurement of S phase duration in human epidermis using cyclin immunostaining and 3H-thymidine pulse labelling. Arch Dermatol Res 284, 238–241 (1992). https://doi.org/10.1007/BF00375801

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  • DOI: https://doi.org/10.1007/BF00375801

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