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Induction and repair of UVB-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in organ-cultured normal human skin

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Summary

To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers and (6-4) photoproducts were observed in a dosedependent manner after UVB irradiation. The intensity of nuclear immunofluorescence of the upper epidermal layers was stronger and clearer than that of the lower epidermal layers. DNA repair time-course studies showed that both types of DNA damage could be repaired within 24 h after UVB irradiation.

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Authors and Affiliations

  1. Department of Dermatology, Nara Medical University, 840 Shijo-cho, Kashihara, 634, Nara, Japan

    T. Muramatsu, N. Kobayashi, H. Tada, M. Yamaji & T. Shirai

  2. RI Center, Nara Medical University, 840 Shijo-cho, Kashihara, 634, Nara, Japan

    T. Mori

  3. Department of Biology, Nara Medical University, 840 Shijo-cho, Kashihara, 634, Nara, Japan

    T. Ohnishi

Authors
  1. T. Muramatsu
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  2. N. Kobayashi
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  3. H. Tada
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  4. M. Yamaji
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  5. T. Shirai
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  6. T. Mori
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  7. T. Ohnishi
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Muramatsu, T., Kobayashi, N., Tada, H. et al. Induction and repair of UVB-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in organ-cultured normal human skin. Arch Dermatol Res 284, 232–237 (1992). https://doi.org/10.1007/BF00375800

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  • DOI: https://doi.org/10.1007/BF00375800

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