Summary
The immunohistochemical localization of basic fibroblast growth factor (bFGF) was examined during wound healing in mouse skin. Frozen sections taken from the rounded skin defects were reacted with polyclonal anti-human recombinant bFGF IgG followed by incubation with FITC-conjugated IgG. The basal layer keratinocytes and hair bulbs at the wound edge were strongly stained with this antibody. In the reepithelized area, several layers of keratinocytes from the basal layer were positively stained regardless of the time after wounding. These findings suggest that germinative keratinocytes which express bFGF function as leading cells in the covering of the wound defect. However, dermal granulation tissue, including capillary endothelial cells, fibroblasts and macrophages unexpectedly did not demonstrate any immunoreactivity throughout the process of wound healing. Simultaneous histochemical investigation using cultivated mouse keratinocytes and bovine aortic endothelial cells showed primarily cytoplasmic fluorescence. The discrepancy in the staining patterns of endothelial cells in vivo and in vitro suggests that immunoreactive bFGF is either not expressed in vivo, or is processed or masked.
Similar content being viewed by others
References
Boyce ST, Ham RG (1983) Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol 81: 33s-40s
DiMario J, Buffinger N, Yamada S, Strohman RC (1989) Fibroblast growth factor in the extracellular matrix of dystrophic mouse muscle. Science 244: 688–690
Folkman J, Klagsbrun M (1987) Angiogenetic factors. Science 235: 442–7
Folkman J, Klagsbrum M, Sasse J, Wadzinski M, Ingber D, Vlodavsky I (1988) A heparin-binding angiogenic protein — basic fibroblast growth factor — is stored within basement membrane. Am J Pathol 130: 393–400
Gonzalez A-M, Buscaglia M, Ong M, Baird A (1990) Distribution of basic fibroblast growth factor in the 18-day rat fetus: localization in the basement membranes of diverse tissues. J Cell Biol 110: 753–765
Gospodarowicz D, Ferrana N, Schweigerer L, Neufeld G (1987) Structual characterization and biological functions of basic fibroblast growth factor. Endocrinol Rev 8: 95–114
Grothe C, Unsicker K (1989) Immunocytochemical localization of basic firoblast growth factor in bovine adrenal gland, ovary and pituitary. J Histochem Cytochem 37: 1877–1883
Halaban R, Langdon R, Birchall N, Cuono C, Baird A, Scott G, Moellmann G. McGuire J (1988) Basic fibroblast growth factor from human keratinocytes is a natural mitogen for melanocytes. J Cell Biol 107: 1611–1619
Hanneken A, Lutty GA, McLeod DS, Robey F, Harvey AK, Hjelmeland LM (1989) Localization of basic fibroblast growth factor to the developing capillaries of the bovine retina. J Cell Physiol 138: 115–120
Joseph-Silverstein J, Consigli SA, Lyser KM, Pault CV (1989) Basic fibroblast growth factor in the chick embryo: immunolocalization to striated muscle cells and their precursors. J Cell Biol 108: 2459–2466
Kalcheim C, Neufeld G (1990) Expression of basic fibroblast growth factor in the nervous system of early avian embryos. Development 109: 203–215
Kardami E, Fandrich RR (1989) Basic fibroblast growth factor in atria and ventricles of the vertebrate heart. J Cell Biol 109: 1865–1875
Lindner V, Reidy MA, Fingerle J (1989) Regrowth of arterial endothelium: Denudation with minimal trauma leads to complete endothelial cell regrowth. Lab Invest 61: 556–563
Pauls W, Grothe C, Sensenbrenner M, Janet T, Baur I, Graf M, Roggendorf W (1990) Localization of basic fibroblast growth factor, a mitogen and angiogenic factor, in human brain tumors. Acta Neuropathol 79: 418–423
Renko M, Quarto N, Morimoto T, Rifkin DB (1990) Nuclear and cytoplasmic localization of different basic fibroblast growth factor species. J Cell Physiol 144: 108–114
Rifkin DB, Moscatelli D (1989) Recent development in the cell biology of basic fibroblast growth factor. J Cell Biol 109: 1–6
Sato Y, Rifkin DB (1988) Autocrine activities of basic fibroblast growth factor: Regulation of endothelial cell movement, plasminogen activator synthesis and DNA synthesis. J Cell Biol 107: 1199–1205
Schulze-Osthoff K, Risau W, Vollmer E, Sorg C (1990) In situ detection of basic fibroblast growth factor by highly specific antibodies. Am J Pathol 137: 85–92
Schulze-Osthoff K, Goerdt S, Sorg C (1990) Expression of basic fibroblast growth factor (bFGF) in Kaposi's sarcoma: an immunohistologic study. J Invest Dermatol 95: 238–240
Sunderkötter C, Roth J, Sorg C (1990) Immunohistochemical detection of bFGF and TNF-α in the course of inflammatory angiogenesis in the mouse cornea. Am J Pathol 137: 511–515
Tsuboi R, Rifkin DB (1990) Recombinant basic fibroblast growth factor stimulates wound healing in healing-impaired db/db mice. J Exp Med 172. 245–251
Zagzag D, Miller DC, Sato Y, Rifkin DB, Burstein DE (1990) Immunohistochemical localization of basic fibroblast growth factor in astrocytomas. Cancer Res 50: 7393–7398
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kurita, Y., Tsuboi, R., Ueki, R. et al. Immunohistochemical localization of basic fibroblast growth factor in wound healing sites of mouse skin. Arch Dermatol Res 284, 193–197 (1992). https://doi.org/10.1007/BF00375792
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00375792