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The enhancement of catecholamine-induced Cl− current by cyclic GMP revealed using photolabile caged compounds in guinea-pig ventricular cells

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  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

Whole cell currents were recorded in single myocytes dissociated from guinea-pig ventricles, and caged compounds were loaded intracellularly through the patch electrodes. Flash photolysis of caged cyclic GMP (cGMP) increased the amplitudes of both catecholamine-induced Cl− (ICl) and Ca2+ currents (ICa) which were pre-activated by submaximum doses of isoprenaline. Transient activation of ICl by photo-release of cyclic AMP (cAMP) showed a half decay time (t1/2) of 16.7±1.4 sec (mean±S.E.M., n=14). This decay was markedly delayed by inhibiting phosphodiesterases using 3-isobutyl-1-methyl-xanthine (IBMX). The intracellular application of cGMP (10–50μ M)also prolonged the decay of the ICl response to caged cAMP (t1/2=38.0±7.1 sec, n=12). These findings strongly support the hypothesis that cGMP facilitates theβ-adrenergic response of ionic currents through the inhibition of phosphodiesterase in mammalian cardiac myocytes.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan

    K. Ono, Y. Nakashima & T. Shioya

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  1. K. Ono
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  2. Y. Nakashima
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  3. T. Shioya
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Ono, K., Nakashima, Y. & Shioya, T. The enhancement of catecholamine-induced Cl− current by cyclic GMP revealed using photolabile caged compounds in guinea-pig ventricular cells. Pflügers Arch. 424, 546–548 (1993). https://doi.org/10.1007/BF00374920

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  • DOI: https://doi.org/10.1007/BF00374920

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