Skip to main content
SpringerLink
Log in

Effects of internal chloride on ATP-sensitive K-channels in mouse pancreatic β-cells

  • Short Communication
  • Molecular and Cellular Physiology
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

The effect of internal Cl− on the K-ATP channel of pancreatic ß-cells was examined. Reducing Cl− from 140mM to 14mM potentiated channel activity (NPo) ∼4.5 fold in 60% of patches but was without effect in 40% of patches. The Ki for channel inhibition by ATP was not changed. The inhibitory effect of Cl− interacted with the stimulatory action of MgGDP. In 140mM Cl−, ImM MgGDP increased NPo in Cl−-sensitive patches only: when NPo was potentiated by 14mM Cl−, no further increase was produced by MgGDP. These observations suggest that MgGDP and low Cl− solution may increase channel activity via a similar mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Ashcroft FM, Ashcroft SJH (1990) Cell Sig 2:197–214

    Google Scholar 

  2. Takano M, Noma A (1993) Prog Neurobiol 41:21–30

    Google Scholar 

  3. Bokvist K, Ämmälä C, Ashcroft FM, Berggren PO, Larsson O, Rorsman P (1991) Proc Roy Soc B 243:139–144

    Google Scholar 

  4. Proks P, Takano M, Ashcroft FM (1994) J Physiol 475:33–44

    Google Scholar 

  5. Tung RT, Kurachi Y (1991) J Physiol 437:239–256

    Google Scholar 

  6. Vaughan-Jones RD (1982) Phil Trans Roy Soc Lond 299:537–548

    Google Scholar 

  7. Jiang C, Agulian S, Haddad GG (1992) J Physiol 448:697–708

    Google Scholar 

  8. Trube G, Hescheler J, Schröter K (1989) In: Secretion and its Control. Rockerfeller University Press. pp83–95

  9. McKillen HC, Davies NW, Beirao PSL, Standen NB, Stanfield PR (1993) J Physiol 467:269P.

  10. Smith PA, Ashcroft FM, Fewtrell CMS (1993) J Gen Physiol 101:767–797.

    Google Scholar 

  11. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Pflügers Archiv 391:85–100

    Google Scholar 

  12. Qin D, Noma A (1988) Am J Physiol 255:H980-H984

    Google Scholar 

  13. Proks P, Ashcroft FM (1993) Pflügers Archiv 424:63–72

    Google Scholar 

  14. Nakajima T, Sugimoto T, Kurachi Y (1992) J Gen Physiol 99:665–682

    Google Scholar 

  15. Sehlin J (1978) Am J Physiol 235:E501-E508

    Google Scholar 

Download references

Author information

Author notes

  1. Makoto Takano

    Present address: Department of Physiology, Faculty of Medicine, Kyoto University, 606, Kyoto, Japan

Authors and Affiliations

  1. University Laboratory of Physiology, Parks road, OX1 3PT, Oxford, UK

    Makoto Takano & Frances M. Ashcroft

Authors
  1. Makoto Takano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frances M. Ashcroft
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takano, M., Ashcroft, F.M. Effects of internal chloride on ATP-sensitive K-channels in mouse pancreatic β-cells. Pflügers Arch 428, 194–196 (1994). https://doi.org/10.1007/BF00374859

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00374859

Key words

Search

Navigation