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Pflügers Archiv

, Volume 429, Issue 6, pp 859–869 | Cite as

Expression of the human sodium/proton exchanger NHE-1 in Xenopus laevis oocytes enhances sodium/proton exchange activity and establishes sodium/lithium countertransport

  • Stefan Busch
  • Birgitta -Christina Burckhardt
  • Winfried Siffert
Original Article Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

We investigated whether the human sodium/proton (Na+/H+) exchanger isoform 1 (NHE-1) can mediate sodium/lithium (Na+/Li+) coutertransport. Using the Xenopus laevis oocyte expression system we determined amiloride-sensitive Li+ uptake, a measure of Na+/H+ exchange, in oocytes injected with water or NHE-1 cRNA. Amiloride-sensitive Li+ uptake was three-to tenfold enhanced over control in NHE-1 cRNA-injected cells and was selectively inhibited by 0.01 μM HOE 694 [i.e. (3-methylsulphonyl-4-piperidinobenzoyl) guanidine methanesulphonate]. The endogenously present Na+/H+ exchanger was insensitive to HOE 694. After acidification of oocytes from pH 7.7 to 6.8, amiloride-sensitive Li+ uptake was four-to tenfold higher in NHE-1 cRNA-injected cells than in controls. Li+ efflux from control oocytes was independent of extracellular Na+, indicating that these cells expressed no measurable Na+/Li+ countertransport activity. In NHE-1 cRNA-injected oocytes, Li+ efflux was distinctly enhanced by extracellular Na+ ions. This Na+-dependent Li+ efflux was inhibited by ethylisopropylamiloride, phloretin and by cytosolic acidification. The data show that expression of the NHE-1 in X. laevis oocytes induces the expression of Na+/Li+ countertransport. The data confirm that Na+/H+ exchange and Na+/Li+ countertransport are mediated by the same transport system.

Key words

Xenopus laevis Oocytes Expression Essential hypertension Diabetic nephropathy pH regulation 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Stefan Busch
    • 1
    • 2
  • Birgitta -Christina Burckhardt
    • 3
  • Winfried Siffert
    • 1
    • 2
  1. 1.Max-Planck-Institut für BiophysikFrankfurtGermany
  2. 2.Institut für PharmakologieUniversitätsklinikumEssenGermany
  3. 3.Zentrum der Physiologie und PathophysiologieGöttingenGermany

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