Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein
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Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 μM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type .
Key wordsPatch-clamp technique Vascular endothelium Na channel Tetrodotoxin
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