Pflügers Archiv

, Volume 428, Issue 1, pp 91–93 | Cite as

Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein

  • Dmitri V. Gordienko
  • Hirokazu Tsukahara
Short Communication Heart Circulation, Respiration and Blood; Environmental and Exercise Physiology


Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 μM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type [2].

Key words

Patch-clamp technique Vascular endothelium Na channel Tetrodotoxin 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cohen-Armon M, Sokolovsky M (1993) Evidence for involvement of the voltage-dependent Na+ channel gating in depolarization-induced activation of G-proteins. J Biol Chem 268: 9824–9838Google Scholar
  2. 2.
    Hille B (ed) (1992) Ionic channels of excitable membranes. Sinauer Inc, Sunderland, MA, USAGoogle Scholar
  3. 3.
    Marsden PA, Goligorsky MS, Brenner BM (1991) Endothelial cell biology in relation to current concepts of vessel wall structure and function. J Am Soc Nephrol 1: 931–948Google Scholar
  4. 4.
    Revest PA, Abbott NJ (1992) Membrane ion channels of endothelial cells. TiPS 13: 404–407Google Scholar
  5. 5.
    Sage SO, Van Breemen C, Cannell MB (1991) Sodium-calcium exchange in cultured bovine pulmonary artery endothelial cells. J Physiol (Lond) 440: 569–580Google Scholar
  6. 6.
    Tsukahara H, Gordienko DV, Goligorsky MS (1993) Continuous monitoring of nitric oxide release from human umbilical vein endothelial cells. Biochem Biophys Res Commun 193: 722–729Google Scholar
  7. 7.
    Tsukahara H, Gordienko DV, Tonshoff B, Gelato MC, Goligorsky MS (1994) Direct demonstration of insulin-like growth factor-I-induced nitric oxide production by endothelial cells. Kidney Int 45: 598–604Google Scholar
  8. 8.
    Watsky MA, Cooper K, Rae JL (1991) Sodium channels in ocular epithelia. Pflügers Arch 419: 454–459Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Dmitri V. Gordienko
    • 1
    • 2
  • Hirokazu Tsukahara
    • 1
  1. 1.Department of MedicineState University of New York at Stony BrookNYUSA
  2. 2.Department of Nerve-Muscle PhysiologyA. A. Bogomoletz Institute of Physiology Ukrainian Academy of SciencesKiev-24Ukraine

Personalised recommendations