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Pflügers Archiv

, Volume 423, Issue 3–4, pp 225–231 | Cite as

Multiple mechanisms of manganese-induced quenching of fura-2 fluorescence in rat mast cells

  • Cristina Fasolato
  • Markus Hoth
  • Reinhold Penner
Molecular and Cellular Physiology

Abstract

Whole-cell patch-clamp recordings of membrane currents and fura-2 measurements of free intracellular calcium concentration ([Ca2+]i) were used to study Mn2+ influx in rat peritoneal mast cells. The calcium-selective current, activated by depletion of intracellular calcium stores (ICRAC for calcium release-activated calcium current), supports a small but measurable Mn2+ current. In the presence of intracellular BAPTA, a Mn2+ current through ICRAC was recorded in isotonic MnCl2 (100 mM) without a significant quenching of fura-2 fluorescence. Its amplitude was 10% of that measured in physiological solution containing 10 mM Ca2+. However, following store depletion, a significant quenching of fura-2 fluorescence could be measured only when intracellular BAPTA was omitted, so that all the incoming Mn2+ could be captured by the fluorescent dye. Two other ionic currents activated by receptor stimulation also induced Mn2+ quenching of fura-2 fluorescence: a small current through non-specific cation channels of 50-pS unitary conductance and a distinct cationic current of large amplitude. In addition to these influx mechanisms, Mn2+ was taken up into calcium stores and was subsequently co-released with Ca2+ by Ca2+-mobilizing agonists.

Key words

Calcium stores Cation channels Manganese quenching Fura-2 Patch clamp Calcium release Activated calcium current 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Cristina Fasolato
    • 1
  • Markus Hoth
    • 1
  • Reinhold Penner
    • 1
  1. 1.Department of Membrane BiophysicsMax-Planck-Institute for Biophysical ChemistryGöttingenGermany

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