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Pflügers Archiv

, Volume 431, Issue 1, pp 28–31 | Cite as

Effect of temperature on spontaneous Ca2+-activated CI currents in rabbit portal vein cells

  • R. M. Helliwell
  • W. A. Large
Original Article Molecular and Cellular Physiology

Abstract

The effect of temperature on the time course of spontaneous transient inward (Ca2+-activated Cl) currents (STICs) was investigated with the perforated patch technique in rabbit portal vein cells. STICs decayed exponentially at temperatures between 17° C and 35° C. The time constant of the decay (τ) was decreased with increasing temperature and had a temperature coefficient, Q10, of 2.6. The temperature sensitivity of τ could be described by the Arrhenius equation which gave an activation energy of 16 kcal/mol. This result indicates that STIC decay is governed by a single first-order reaction and provides further support for the hypothesis that the decay is due to the gating properties of Ca2+-activated Cl channels.

Key words

Smooth muscle Ca2+-activated Cl current Temperature 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • R. M. Helliwell
    • 1
  • W. A. Large
    • 1
  1. 1.Department of Pharmacology and Clinical PharmacologySt. George's Hospital Medical SchoolLondonUK

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