Pflügers Archiv

, Volume 422, Issue 4, pp 401–403

Bradykinin stimulation of electrogenic ion transport in epithelial layers of cultured human endometrium

  • C. Jane Matthews
  • Gordon T. A. McEwan
  • Christopher P. F. Redfern
  • Eric J. Thomas
  • Barry H. Hirst
Short Communication Molecular and Cellular Physiology

Abstract

Primary cultures of glandular endometrial epithelial cells grown on permeable supports formed monolayers with a high transepithelial electrical resistance [1096±83 Ω.cm2 (n=34)] and displayed electrogenic ion transport as demonstrated by an inward short circuit current (Isc; 20±2μA/cm2). Bradykinin, 10−8–10−6M, added to either the basolateral or apical solutions enhanced the inward Isc. The concentration-response curves for bradykinin were bell-shaped in nature. The Isc response was more sensitive to apical addition of bradykinin and the maximum response was also greater with apical bradykinin. The increases in Isc were accompanied by two- to three-fold increases in transepithelial conductance. Apical addition of amiloride, 10−4M, reduced the unstimulated Isc by 80%. In the presence of amiloride, the response to both apical and basolateral bradykinin was reduced by >50 % in 8 out of 18 layers, and the mean response was reduced by approximately 25%. The data are consistent with a physiological role for bradykinin in the control of the intra-uterine electrolyte environment, mediated in part by enhanced Na+ absorption.

Key words

bradykinin endometrium epithelia ion transport uterus 

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References

  1. 1.
    Beesley JS, Franks S, Bonney RC (1991) Effect of bradykinin (BK) on human endometrial cell function in vitro. J Reprod Fert Abstr Ser 7:40Google Scholar
  2. 2.
    Clarke LL, Paradiso AM, Mason SJ, Boucher RC (1992) Effects of bradykinin on Na+ and Cl transport in human nasal epithelium. Am J Physiol 262:C644–655Google Scholar
  3. 3.
    Cuthbert AW, Kirkland SC, MacVinish LJ (1985) Kinin effects on ion transport in monolayers of HCA-7 cells, a line from a human colonic adenocarcinoma. Br J Pharmacol 86:3–5Google Scholar
  4. 4.
    Endo T, Fukue H, Kanaya M, Mizunuma M, Fujii M, Yamaoto H, Tanaka S, Hashimoto M (1991) Bombesin and bradykinin increase inositol phosphates and cytosolic free Ca2+, and stimulate DNA synthesis in human endometrial stromal cells. J Endocrinol 131:313–318Google Scholar
  5. 5.
    Matthews CJ, McEwan GTA, Redfern CPF, Thomas EJ, Hirst BH (1992) Electrical properties of primary monolayer cultures of human endometrial glandular epithelial cells. J Physiol 446:97PGoogle Scholar
  6. 6.
    Matthews CJ, Redfern CPF, Hirst BH, Thomas EJ (1992) Characterization of human purified epithelial and stromal cells from endometrium and endometriosis in tissue culture. Fertil Steril 57:990–997Google Scholar
  7. 7.
    Matthews CJ, Redfern CPF, Thomas EJ, Hirst BH (1992) Electrolyte transport by primary monolayer cultures of human endometrial glandular epithelial cells. J Physiol 452:95PGoogle Scholar
  8. 8.
    Ritter M, Lang F (1991) Effect of bradykinin, ATP and adrenaline on cell membrane resistances of Madin-Darby canine kidney cells. J Physiol 443:45–54.Google Scholar
  9. 9.
    Simmons NL (1992) Acetylcholine and kinin augmentation of Cl secretion stimulated by prostaglandin in a canine renal epithelial cell line. J Physiol 447:1–15.Google Scholar
  10. 10.
    Smith JJ, McCann JD, WelshMJ (1990) Bradykinin stimulates airway epithelial Cl secretion via two second messenger pathways. Am J Physiol 258:L369–377Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • C. Jane Matthews
    • 1
  • Gordon T. A. McEwan
    • 1
  • Christopher P. F. Redfern
    • 1
    • 2
  • Eric J. Thomas
    • 1
    • 3
  • Barry H. Hirst
    • 1
  1. 1.Department of Physiological SciencesUniversity of Newcastle upon Tyne Medical SchoolNewcastle upon TyneUK
  2. 2.Medical Molecular Biology Group, Medical SchoolUniversity of Newcastle upon TyneUK
  3. 3.Department of Obstetrics and GynaecologyUniversity of SouthamptonUK

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