Pflügers Archiv

, Volume 422, Issue 4, pp 401–403

Bradykinin stimulation of electrogenic ion transport in epithelial layers of cultured human endometrium

  • C. Jane Matthews
  • Gordon T. A. McEwan
  • Christopher P. F. Redfern
  • Eric J. Thomas
  • Barry H. Hirst
Short Communication Molecular and Cellular Physiology

DOI: 10.1007/BF00374298

Cite this article as:
Matthews, C.J., McEwan, G.T.A., Redfern, C.P.F. et al. Pflügers Arch. (1993) 422: 401. doi:10.1007/BF00374298

Abstract

Primary cultures of glandular endometrial epithelial cells grown on permeable supports formed monolayers with a high transepithelial electrical resistance [1096±83 Ω.cm2 (n=34)] and displayed electrogenic ion transport as demonstrated by an inward short circuit current (Isc; 20±2μA/cm2). Bradykinin, 10−8–10−6M, added to either the basolateral or apical solutions enhanced the inward Isc. The concentration-response curves for bradykinin were bell-shaped in nature. The Isc response was more sensitive to apical addition of bradykinin and the maximum response was also greater with apical bradykinin. The increases in Isc were accompanied by two- to three-fold increases in transepithelial conductance. Apical addition of amiloride, 10−4M, reduced the unstimulated Isc by 80%. In the presence of amiloride, the response to both apical and basolateral bradykinin was reduced by >50 % in 8 out of 18 layers, and the mean response was reduced by approximately 25%. The data are consistent with a physiological role for bradykinin in the control of the intra-uterine electrolyte environment, mediated in part by enhanced Na+ absorption.

Key words

bradykinin endometrium epithelia ion transport uterus 

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • C. Jane Matthews
    • 1
  • Gordon T. A. McEwan
    • 1
  • Christopher P. F. Redfern
    • 1
    • 2
  • Eric J. Thomas
    • 1
    • 3
  • Barry H. Hirst
    • 1
  1. 1.Department of Physiological SciencesUniversity of Newcastle upon Tyne Medical SchoolNewcastle upon TyneUK
  2. 2.Medical Molecular Biology Group, Medical SchoolUniversity of Newcastle upon TyneUK
  3. 3.Department of Obstetrics and GynaecologyUniversity of SouthamptonUK

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