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Dissociation of the effects of training on oxidative metabolism, glucose utilisation and GLUT4 levels in skeletal muscle of streptozotocin-diabetic rats

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Abstract

The effects of long-term, moderate physical exercise on in vivo glucose uptake, levels of two glucose transporter proteins (GLUT1 and GLUT4) and activities of various key enzymes of energy metabolism were measured in skeletal muscle from streptozotocin-diabetic rats. Diabetes (12–16 weeks) reduced the in vivo glucose uptake (glucose metabolic index, GMI) in muscle containing mainly type I fibres by 55% but had no effect in muscles containing mainly type IIa and IIb fibres. GMI was increased in the diabetic white skeletal muscle (mainly type IIb fibres) by more than 120%. In contrast to the complex changes in GMI, GLUT4 levels were reduced in all types of skeletal muscle from diabetic rats with no change in GLUT1 levels. Exercise training had no effects on GMI or the glucose transporter levels. Streptozotocin induced diabetes significantly reduced the oxidative capacity of skeletal muscle assayed as the activities of citrate synthase, succinate dehydrogenase and cytochrome c oxidase. Training increased the activities of oxidative enzymes, with this increase being more prominent in the diabetic animals. The present data indicate that long-term streptozotocin-induced diabetes decreases oxidative metabolic capacity and GLUT4 protein levels in skeletal muscle, but that the changes of glucose transport largely depend on the fibre type composition. Moderate training fully reverses the effect of insulinopenia and hyperglycaemia on muscle oxidative metabolism. In contrast to the previous suggestions, the expression of GLUT4 is not correlated with the capacity of oxidative metabolism in skeletal muscle of streptozotocin-diabetic rats.

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Authors and Affiliations

  1. Department of Clinical Medicine, University of Tampere, Box 607, SF-33101, Tampere, Finland

    Heikki Kainulainen

  2. Department of Pharmacology and Toxicology, RWTH Aachen, Wendlingweg 2, D-52074, Aachen, Germany

    Heikki Kainulainen & Hans G. Joost

  3. LIKES-Research Centre, University Campus, SF-40100, Jyväskylä, Finland

    Jyrki Komulainen & Veikko Vihko

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  1. Heikki Kainulainen
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  2. Jyrki Komulainen
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  3. Hans G. Joost
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  4. Veikko Vihko
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Kainulainen, H., Komulainen, J., Joost, H.G. et al. Dissociation of the effects of training on oxidative metabolism, glucose utilisation and GLUT4 levels in skeletal muscle of streptozotocin-diabetic rats. Pflügers Arch. 427, 444–449 (1994). https://doi.org/10.1007/BF00374259

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  • DOI: https://doi.org/10.1007/BF00374259

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