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Pflügers Archiv

, Volume 422, Issue 6, pp 609–613 | Cite as

A method for rapid exchange of solutions at membrane patches using a 10-μl microcapsule

  • Duk -Su Koh
  • Werner Vogel
Instruments and Techniques Molecular and Cellular Physiology

Abstract

A rapid exchange (less than 2 ms) of the bath solution facing a membrane patch is accomplished by driving the tip of a pipette from the bath through a 100-μm oil layer into a small capsule filled with 10 μl test solution. The microcapsule method can be applied to both excised patch configurations, inside-out and outside-out patches. On and off reactions of Ca2+-activated K+ channel activity have been recorded after changing the intracellular Ca2+ concentration using an inside-out patch. A blockade of these K+ channels by external tetraethylammonium ions is demonstrated with an outside-out patch. The blocking kinetics of delayed-rectifier K+ channels by a purified peptide toxin from snake venom, dendrotoxin, could be measured with our microcapsule method. Using tiny volumes of test solutions this method can be helpful in experiments involving scarce or expensive solutions.

Key words

Oil-gated microcapsule Rapid exchange of solution Patch clamp Blockade of ion channel Myelinated nerve fibre 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Duk -Su Koh
    • 1
  • Werner Vogel
    • 1
  1. 1.Physiologisches InstitutJustus-Liebig-UniversitätGiessenGermany

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