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Pflügers Archiv

, Volume 422, Issue 6, pp 591–598 | Cite as

Activation of the nicotinic acetylcholine receptor mobilizes calcium from caffeine-insensitive stores in C2C12 mouse myotubes

  • F. Grassi
  • A. Giovannelli
  • S. Fucile
  • F. Eusebi
Excitable Tissues and Central Nervous Physiology

Abstract

In cultured mouse C2C12 myotubes, digital Ca2+ imaging fluorescence microscopy using the acetoxymethyl ester of Fura-2, Fura-2-AM, showed that, in the absence of extracellular Ca2+, acetylcholine (ACh) and nicotine, but not muscarine, raised the intracellular concentration of Ca2+ ([Ca2+]i) by about tenfold. AChinduced Ca2+ mobilization was prevented by thapsigargin, a drug known to deplete inositol 1,4,5-trisphosphate (InsP3)-sensitive stores, and was concomitant with InsP3 accumulation. Caffeine, which releases Ca2+ from the ryanodine-sensitive stores of the sarcoplasmic reticulum, did not interfere with the ACh-induced [Ca2+]i increase. Ca2+ mobilization was also inhibited when myotubes were depolarized by high K+, or when extracellular Na+ was omitted. Nicotinic ACh receptor (nAChR) stimulation lowered intracellular pH with a time course slower than the [Ca2+]i increase. Possible mechanisms linking the current flowing through the nAChR pore to [Ca2+]i increase are discussed.

Key words

Calcium Acetylcholine Nicotine Nicotinic acetylcholine receptor C2C12 myotubes Inositol 1,4,5-trisphosphate 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • F. Grassi
    • 1
  • A. Giovannelli
    • 2
  • S. Fucile
    • 1
  • F. Eusebi
    • 1
  1. 1.Department of Experimental MedicineUniversity of RomeRomeItaly
  2. 2.Department of Experimental MedicineUniversity of L'AquilaL'AquilaItaly

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