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Pflügers Archiv

, Volume 422, Issue 6, pp 564–569 | Cite as

Arterial size determines the enhancement of contractile responses after suppression of endothelium-derived relaxing factor formation

  • Jan Galle
  • Johann Bauersachs
  • Eberhard Bassenge
  • Rudi Busse
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology

Abstract

We studied the effect of endothelium-derived relaxing factor (EDRF) on norepinephrine-induced contractile responses and on the tissue guanosine-3′,5′-phosphate (cGMP) concentration of isolated rabbit arteries with an increasing endothelium to smooth muscle cell ratio (aorta, femoral and mesenteric arteries). After suppression of EDRF formation (either by NG-nitro-l-arginine or, in mesenteric arteries, by saponin), contractions elicited by cumulative doses of norepinephrine were unaltered in aorta but were enhanced by 22.5% in femoral arteries and by 44.3% in mesenteric arteries (at the highest norepinephrine concentration). The cGMP concentration (pmol/mg protein) of unstimulated, endotheliumintact vessels decreased after suppression of EDRF formation from 1.09±0.24 to 0.74±0.28 in aortic, from 2.86±0.4 to 0.61±0.19 in femoral and from 6.3±0.9 to 0.7±0.15 in mesenteric arterial segments. The basal cGMP concentration did not differ in endothelium-denuded segments of these arteries, suggesting a similar basal activity of soluble guanylate cyclase (sGC). A higher sensitivity of sGC may have contributed to the higher cGMP concentration observed in the smaller arteries, since in the presence of sodium nitroprusside the cGMP concentration of endothelium-denuded segments increased 1.8-fold in aortic, 2.9-fold in femoral and 2.4 fold in mesenteric arterial segments. However, these differences in sGC activation cannot be solely responsible for the high basal cGMP concentration in endotheliumintact mesenteric arteries. The greater ratio of endothelium to smooth muscle cell layers in the smaller arteries might result in a higher EDRF concentration in the vascular wall and subsequently in a higher cGMP concentration. In conclusion, these data support the view of a greater importance of EDRF-mediated vascular control in small arteries than in large conduit arteries.

Key words

Vascular reactivity Endothelium Smooth muscle cells cGMP NG-nitro-l-arginine Endothelium-derived relaxing factor (EDRF) Nitric oxide 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jan Galle
    • 1
  • Johann Bauersachs
    • 2
  • Eberhard Bassenge
    • 2
  • Rudi Busse
    • 2
  1. 1.Department of Medicine IVUniversity of FreiburgFreiburgGermany
  2. 2.Department of Applied PhysiologyUniversity of FreiburgFreiburgGermany

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