Abstract
Administration of β-guanidinopropionic acid (β-GPA) to rats as 1% of their diet for 6 weeks led to an accumulation of β-GPA and β-GPA-phosphate and to a depletion of creatine and phosphocreatine in the fast-twitch plantaris muscle. Adenosine triphosphate concentration was also decreased. Electrophoretic analyses were performed to investigate the effects of β-GPA on the patterns of fast (FM) and slow (SM) isomyosins, myosin heavy chain (HC) isoforms and myosin light chain (LC) isoforms. The relative concentrations of fast isomyosins FM1 and FM2 decreased, whereas slow isomyosin SM increased. The increase in slow isomyosin corresponded to an increase in the relative concentration of the slow myosin HCI. The changes of the myosin light chain pattern consisted of increases in the relative concentrations of the two slow isoforms, LC1sb and LC2s, and decreases in the fast isoforms LC2f and LC3f. These results demonstrate that β-GPA administration, leading to a depletion in energy-rich phosphates and a reduced phosphorylation potential, has an impact on myosin isoform expression in rat fast-twitch skeletal muscle.
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Ren, J.M., Ohira, Y., Holloszy, J.O. et al. Effects of β-guanidinopropionic acid-feeding on the patterns of myosin isoforms in rat fast-twitch muscle. Pflügers Arch 430, 389–393 (1995). https://doi.org/10.1007/BF00373914
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DOI: https://doi.org/10.1007/BF00373914