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On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation

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  • Molecular and Cellular Physiology
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Abstract

The Ca2+ channel subunits α1C-a and α1C-b were stably expressed in Chinese hamster ovary (CHO) and human embryonic kidney (HEK) 293 cells. The peak Ba2+ current (I Ba) of these cells was not affected significantly by internal dialysis with 0.1 mM cAMP-dependent protein kinase inhibitor peptide (mPKI), 25 μM cAMP-dependent protein kinase catalytic subunit (PKA), or a combination of 25 μM PKA and 1 μM okadaic acid. The activity of the α1C-b channel subunit expressed stably in HEK 293 cells was depressed by 1 μM H 89 and was not increased by superfusion with 5 μM forskolin plus 20 μM isobutylmethylxanthine (IBMX). The α1C-a·β2·α2/δ complex was transiently expressed in HEK 293 cells; it was inhibited by internal dialysis of the cells with 1 μM H 89, but was not affected by internal dialysis with mPKI, PKA or microcystin. Internal dialysis of cells expressing the α1C-a·β2·α2/δ channel with 10 μM PKA did not induce facilitation after a 150-ms prepulse to +50 mV. The Ca2+ current (I Ca) of cardiac myocytes increased threefold during internal dialysis with 5 μM PKA or 25 μM microcystin and during external superfusion with 0.1 μM isoproterenol or 5 μM forskolin plus 50 μM IBMX. These results indicate that the L-type Ca2+ channel expressed is not modulated by cAMP-dependent phosphorylation to the same extent as in native cardiac myocytes.

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Author notes

  1. Eva Bosse & Veit Flockerzi

    Present address: Pharmakologisches Institut, Abteilung Molekulare Pharmakologie, Medizinische Fakultät, Universität Heidelberg, Im Neuenheimer Feld 366, D-69120, Heidelberg, Germany

Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der TUM, Biedersteiner Strasse 29, D-80802, Munich, Germany

    Xiangang Zong, Jürgen Schreieck, Gerhard Mehrke, Andera Welling, Angela Schuster, Eva Bosse, Veit Flockerzi & Franz Hofmann

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  1. Xiangang Zong
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  2. Jürgen Schreieck
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  6. Eva Bosse
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  7. Veit Flockerzi
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  8. Franz Hofmann
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Zong, X., Schreieck, J., Mehrke, G. et al. On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation. Pflügers Arch 430, 340–347 (1995). https://doi.org/10.1007/BF00373908

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  • DOI: https://doi.org/10.1007/BF00373908

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