Abstract
A Ca2+ current activated by store depletion has been described recently in several cell types and has been termed I CRAC (for Ca2+ release-activated Ca2+ current). In this paper, the Ca2+ and Ba2+ permeability of CRAC channels is investigated in mast cells, rat basophilic leukaemia cells (RBL) and human T-lymphocytes (Jurkat). The selectivity of CRAC channels for Ca2+ over monovalent cations is identical in all three cell types and is at least as high as that of voltage-operated Ca2+ (VOC) channels in the various tissues tested. The amplitude of Ba2+ currents relative to Ca2+ currents (I Ba/I Ca) through CRAC channels was found to be strongly dependent on the membrane potential and was much smaller in Jurkat cells compared to mast and RBL cells. An anomalous mole-fraction behavior was observed at very negative membrane potentials in all three cell types when using different mixtures of external Ca2+ and Ba2+. In contrast to VOC channels, the anomalous mole-fraction effect was not observed at potentials positive to−20 mV.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Almers W, McCleskey EW (1984) Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore. J Physiol (Lond) 353:585–608
Armstrong CM, Neyton J (1991) Ion permeation through calcium channels: a one-site model. Calcium entry and action at the presynaptic nerve terminal. Ann NY Acad Sci 635:18–25
Bahnson T, Pandol SJ, Dionne VE (1993) Cyclic GMP mediates depletion-activated Ca2+ entry in pancreatic acinar cells. J Biol Chem 268:10808–10812
Bezanilla F, Armstrong CM (1972) Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons. J Gen Physiol 60:588–608
Eckert R, Chad JE (1984) Inactivation of Ca channels. Prog Biophys Mol Biol 44:215–267
Fasolato C, Hoth M, Penner R (1993) A GTP-dependent step in the activation mechanism of capacitative calcium entry. J Biol Chem 268:20737–20740
Fasolato C, Innocenti B, Pozzan T (1994) Receptor-activated Ca2+ influx: how many mechanisms for how many channels. Trends Pharmacol Sci 15:77–83
Fenwick EM, Marty A, Neher E (1982) Sodium and calcium channels in bovine chromaffiin cells. J Physiol (Lond) 331:599–635
Friel DD, Tsien RW (1989) Voltage-gated calcium channels: direct observation of the anomalous mole fraction effect at the single-channel level. Proc Natl Acad Sci USA 86:5207–5211
Hess P, Tsien RW (1984) Mechanism of ion permeation through calcium channels. Nature 309:453–456
Hoth M, Penner R (1992) Depletion of intracellular calcium stores activates a calcium current in mast cells. Nature 355:353–356
Hoth M, Penner R (1993) Calcium release-activated calcium current in rat mast cells. J Physiol (Lond) 465:359–386
Hoth M, Penner R (1994) Complex permeability pattern of the capacitative calcium entry mechanism ICRAC in defferent non excitable cells (abstract). Pflügers Arch 426:R30
Kostyuk PG, Mironov SL, Shuba YAM (1983) Two ion-selecting filters in the calcium channel of the somatic membrane of mollusc neurons. J Membr Biol 76:83–93
Lee KS, Tsien RW (1984) High selectivity of calcium channels in single dialyzed heart cells of the guinea-pig. J Physiol (Lond) 430:150–188
Lewis RS, Cahalan MD (1989) Mitogen-induced oscillations of cytosolic Ca2+ and transmembrane Ca2+ current in human leukemic T cells. Cell Regul 1:99–112
Lückhoff A, Clapham DE (1994) Calcium channels activated by depletion of internal calcium stores in A431 cells. Biophys J 67:177–182
Neher E, Augustine GJ (1992) Calcium gradients and buffers in bovine chromaffin cells. J Physiol (Lond) 450:273–301
Parekh AB, Terlau H, Stühmer W (1993) Depletion of InsP3 stores activates a Ca2+ and K+ current by means of a phosphatase and a diffusible messenger. Nature 364:814–818
Premack BA, McDonald TV, Gardner P (1994) Activation of Ca2+ current in Jurkat T cells following the depletion of Ca2+ stores by microsomal Ca2+-ATPase inhibitors. J Immunol 152:5226–5240
Putney JW (1986) A model for receptor-regulated calcium entry. Cell Calcium 7:1–12
Seifert W, Ranscht B, Finck HJ, Förster F, Beckh S, Möller HW (1983) Combined fura-2 and patch clamp measurements in rat peritoneal mast cells. In: Seifert W (ed) Neurobiology of the hippocampus. Academic, London, pp 109–135
Vaca L, Kunze DL (1994) Depletion of intracellular Ca2+ stores activates a Ca2+-selective channel in vascular endothelium. Am J Physiol 267:C920-C925
Vaca L, Sinkins WG, Hu Y, Kunze DL, Schilling WP (1994) Activation of recombinant trp by thapsigargin in Sf9 insect cells. Am J Physiol 267:C1501-C1505
Yue DT, Marban E (1990) Permeation in the dihydropyridine-sensitive calcium channel. J Gen Physiol 95:911–939
Zhou Z, Neher E (1994) Calcium permeability of nicotinic acetylcholine receptor channels in bovine adrenal chromaffin cells. Pflügers Arch 425:511–517
Zweifach A, Lewis RS (1993) The mitogen-regulated calcium current of T lymphocytes is activated by depletion of intracellular calcium stores. Proc Natl Acad Sci USA 90:6295–6299
Zweifach A, Lewis RS (1995) Rapid inactivation of depletion-activated calcium current (I CRAC) due to local calcium feedback. J Gen Physiol (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hoth, M. Calcium and barium permeation through calcium release-activated calcium (CRAC) channels. Pflügers Arch 430, 315–322 (1995). https://doi.org/10.1007/BF00373905
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00373905