Skip to main content
SpringerLink
Log in

Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts

  • Short Communication
  • Molecular and Cellular Physiology
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than −28±4 mV from a holding potential of −80 mV. It peaked within 1–1.5 ms, and inactivated within 3.3–6.9 ms. The 50% inactivation voltage was −59±6 mV with a steepness factor of 0.11±0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 < 15 nM) but not by verapamil (≤100μM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Chesnoy-Marchais, D. and Fritsch, J. (1988) J. Physiol. 398: 291–311

    PubMed  Google Scholar 

  2. Gordienko, D.V., Tsukahara, H. (1994) Pflügers Arch. 428: 91–93

    Google Scholar 

  3. Hille, B. (1991) Ionic channels of excitable membranes, Sinauer, Sunderland, Mass.

    Google Scholar 

  4. Kelly, M.E.M., Dixon, S.J., and Sims, S.M. (1992) J. Membrane Biol. 126: 171–181

    Article  Google Scholar 

  5. Miyauchi, A., Hruska, K.A., Greenfield, E.M., Duncan, R., Alvarez, J., Barattolo, R., Colucci, S., Zambonin-Zallone, A., Teitelbaum, S.L., and Teti, A. (1990) J. Cell Biol. 111: 2543–2552

    Article  PubMed  Google Scholar 

  6. Ogata, N. and Tatebayashi, H. (1993) J. Physiol. 466: 9–37

    PubMed  Google Scholar 

  7. Ravesloot, J.H., Ypey, D.L., Vrijheid-Lammers, T., and Nijweide, P.J. (1989) Proc. Natl. Acad. Sci. U.S.A. 86: 6821–6825

    PubMed  Google Scholar 

  8. Wen, R., Lui, G.M. and Steinberg, R.H. (1994) J. Physiol. 476: 187–196

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biophysics Department, University Medical School, H-4012, Debrecen, Hungary

    Rezső Gáspár Jr. & Zoltán Krasznai

  2. Cell Biology and Histology Departments, Leiden University, 2300, RC Leiden, The Netherlands

    A. Freek Weidema & Peter J. Nijweide

  3. Physiology and Physiological Physics, Leiden University, 2300, RC Leiden, The Netherlands

    A. Freek Weidema & Dirk L. Ypey

Authors
  1. Rezső Gáspár Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Freek Weidema
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zoltán Krasznai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peter J. Nijweide
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dirk L. Ypey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gáspár, R., Weidema, A.F., Krasznai, Z. et al. Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts. Pflugers Arch. 430, 596–598 (1995). https://doi.org/10.1007/BF00373899

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00373899

Key words

Search

Navigation