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Ionic basis of neurokinin-A-induced depolarization in single smooth muscle cells isolated from guinea-pig trachea

  • Original Article
  • Molecular and Cellular Physiology
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Abstract

Neurokinin A (NKA) caused single tracheal smooth muscle cells (TSMCs) to contract. The effects of NKA on the electrical activity of guinea-pig TSMCs were examined using the tight-seal whole-cell patch-clamp technique. Under current-clamp conditions at rest, the membrane potential of TSMCs spontaneously oscillated at about −40 mV and NKA rapidly depolarized the membrane potential to nearly 0 mV, which then gradually repolarized to about −20 mV in the presence of NKA. The oscillations in potential disappeared transiently during the rapid phase of depolarization in response to NKA and reappeared during the sustained phase of depolarization. Under voltage-clamp conditions, NKA evoked an inward current which faded quickly. Subsequently, the cell conductance in the presence of NKA at potentials greater than −40 mV decreased gradually. The reversal potential of the NKA-induced inward current was about 0 mV, and shifted with changes in the Cl− equilibrium potential. The Cl− current was not elicited by NKA when using a pipette solution containing 10 mM ethylenebis(oxonitrilo) tetraacetic acid (EGTA). During the sustained phase, K+ currents evoked by depolarizing voltage steps were inhibited by NKA. The present results indicate that NKA causes rapid and sustained depolarization of TSMCs by two distinct mechanisms: (1) initial transient activation of the Ca2+-dependent Cl− current, and (2) sustained inhibition of K+ currents.

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Authors and Affiliations

  1. The Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Toshiaki Nakajima, Hisanori Hazama, Eiji Hamada & Masao Omata

  2. Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, Mayo Foundation, 55905, Rochester, MN, USA

    Yoshihisa Kurachi

  3. Department of Pharmacology II, Faculty of Medicine, Osaka University, Yamadaoka 2-2, Suita, 565, Osaka, Japan

    Yoshihisa Kurachi

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  1. Toshiaki Nakajima
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  4. Masao Omata
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Nakajima, T., Hazama, H., Hamada, E. et al. Ionic basis of neurokinin-A-induced depolarization in single smooth muscle cells isolated from guinea-pig trachea. Pflugers Arch. 430, 552–562 (1995). https://doi.org/10.1007/BF00373892

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  • DOI: https://doi.org/10.1007/BF00373892

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