Abstract
Changes in activity and abundance of renal Na,K-ATPase were evaluated during cold exposure and hibernation of the jerboa Jaculus orientalis by measuring the hydrolytic activity, the number of units and the transport activity of Na,K-ATPase in isolated nephron segments. As compared to controls, jerboas exposed to cold (6 °C) for 4–5 weeks displayed mild diuresis, decreased urinary osmolality and increased kaliuresis. In cold-exposed jerboas, Na,K-ATPase hydrolytic activity was reduced in the medullary thick ascending limb and enhanced in the cortical and outer medullary collecting duct, whereas it was not altered in other nephron segments. The number of Na,K-ATPase units and the activity of Na,K-pump, determined by [3H]-ouabain binding and by ouabain-sensitive rubidium uptake respectively, changed in parallel with the hydrolytic activity in the medullary thick ascending limb and cortical collecting duct. The maximal rate of activity (V max) of Na,K-ATPase was not modified further during hibernation. Thus, cold exposure, but not the onset of hibernation, induces segment-specific changes in the abundance and activity of Na,K-ATPase units which are likely to be related to the entry into hibernation, but not to the maintenance of some renal functions during deep hibernation.
Similar content being viewed by others
References
Bowler K, Duncan CJ (1969) The temperature characteristics of brain microsomal ATPase of the hedgehog: changes associated with hibernation. Physiol Zool 42:211–219
Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Charnock JS, Simonson LP (1978) Variations in (Na+ + K+)-ATPase and Mg2+-ATPase activity of the ground squirrel brain during hibernation. Comp Biochem Physiol [B]59:223–229
Charnock JS, Simonson LP (1978) Seasonal variations in the renal cortical (Na+ + K+)-ATPase and Mg2+-ATPase of a hibernator, the ground squirrel (Spermophilus richardsonii). Comp Biochem Physiol [B]60:433–439
Chatfield P, Battista A, Lyman C, Garcia J (1948) Effects of cooling on nerve conduction in a hibernator (golden hamster) and non-hibernator (albino rat). Am J Physiol 155:179–185
Cheval L, Doucet A (1990) Measurement of Na-K-ATPase-mediated rubidium influx in single segments of rat nephron. Am J Physiol 259:F111-F121
Doucet A, Katz AI, Morel F (1979) Determination of Na-K-ATPase activity in single segments of the mammalian nephron. Am J Physiol 237:F105-F113
Doucet A, Barlet C, Baddouri K (1987) Effect of water intake on Na-K-ATPase in nephron segments of the desert rodent, Jaculus orientalis. Pflügers Arch 408:129–132
Dunnett CW (1955) A multiple comparison procedure for comparing several treatments with a control. J Am Stat Assoc 50:1096–1121
El Mernissi G, Doucet A (1984) Quantitation of [3H]ouabain binding and turnover of Na-K-ATPase along the rabbit nephron. Am J Physiol 247:F158-F167
Fang LST, Willis JS (1974) Increase of Na-K-ATPase activity in renal cortex of hamster (Mesocricetus auratus) during prehibernation cold exposure. Comp Biochem Physiol [A]48:687–698
Fiske CH, SubbaRow Y (1925) The colorimetric determination of phosphorus J Biol Chem 66:375–400
South FE (1961) Phrenic nerve-diaphragm preparations in relation to temperature and hibernation. Am J Physiol 200:565–571
Willis JS (1966) Characteristics of ion transport in kidney cortex of mammalian hibernators. J Gen Physiol 49:1221–1239
Willis JS (1968) Cold resistance of kidney cells of mammalian hibernators: cation transport vs. respiration. Am J Physiol 214:923–928
Willis JS, Li NM (1969) Cold resistance of Na,K-ATPase of renal cortex of the hamster, a hibernating mammal. Am J Physiol 217:321–326
Zeidler RB, Willis JS (1976) Cultured cells from renal cortex of hibernators and nonhibernators. Regulation of cell K+ at low temperature. Biochim Biophys Acta 436:628–651
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bennis, C., Cheval, L., Barlet-Bas, C. et al. Effects of cold exposure and hibernation on renal Na,K-ATPase of the jerboa Jaculus orientalis . Pflugers Arch. 430, 471–476 (1995). https://doi.org/10.1007/BF00373883
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00373883