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Properties of the nicotinic-receptor-activated current in adrenal chromaffin cells of the guinea-pig

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Abstract

Properties of acetylcholine(ACh)- and nicotine-induced currents were studied in the guinea-pig chromaffin cell, using the whole-cell and cell-attached versions of the patch-clamp technique. Bath application of ACh or nicotine, but not muscarine, produced an inward current associated with an increase in current noise at a membrane potential of −70 mV. Low concentrations of both agonists produced a sustained inward current whereas high concentrations produced a transient, then a sustained inward current. Nicotine was about twice as potent as ACh in inducing the inward current. Hexamethonium (6 μM) inhibited the ACh-induced current but not in a competitive manner. By contrast, atropine (6 μM) inhibited the ACh-induced current more strongly with increasing concentrations of ACh. The nicotinic-receptor-activated current (“nicotinic current”) showed inward rectification and, when Cs+ was used instead of K+ in the pipette solution, the polarity of the current changed at around −5 mV and a negative slope occurred between +10 mV and +30 mV. The nicotinic channel had a unit conductance of 33 pS. During the initial 20–30 min of whole-cell voltage-clamp recording, the time course of the agonist-induced desensitization was markedly facilitated. Addition of 5 mM MgATP in the pipette solution at least partly prevented this facilitation of the desensitization. The frequency of activation of the nicotinic receptor and the extracellular Ca2+ were not primary factors in the acceleration of rate of desensitization.

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Author notes

  1. Masumi Inoue

    Present address: Department of Physiology, Faculty of Medicine, Fukuoka University, 814-01, Fukuoka, Japan

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  1. Department of Pharmacology, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan

    Masumi Inoue & Hirosi Kuriyama

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  1. Masumi Inoue
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  2. Hirosi Kuriyama
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Inoue, M., Kuriyama, H. Properties of the nicotinic-receptor-activated current in adrenal chromaffin cells of the guinea-pig. Pflügers Arch. 419, 13–20 (1991). https://doi.org/10.1007/BF00373741

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  • DOI: https://doi.org/10.1007/BF00373741

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