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Effect of shear stress on activity of cellular enzyme in animal cell

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Abstract

The effect of shear stress on the activity of cellular enzyme in an animal cell was discussed by using a flow channel. The activity of lactate dehydrogenase (LDH) in cells exposed to a shear stress of 0.5 Pa for 12 h was about 4-fold greater than in the cells without exposure to shear stress. The relative LDH activity was correlated with the dissipation energy density of the flowing medium. A good correlation was obtained and it was found that the dependency of cellular enzyme activity on the shear stress and the exposure time was related to the transmission of the energy from the flowing medium to attached cells.

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Abbreviations

b m:

width of flow channel

E J/m2 :

dissipation energy density

h m:

distance between two plates

Q m3/s:

volume flow rate

t s:

exposure time

u m/s:

velocity of medium

y m:

distance from wall attached cells

μ Pa s:

viscosity

τ Pa:

shear stress

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Authors and Affiliations

  1. Department of Bioengineering Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, 4259, Yokohama, Japan

    N. Shiragami & H. Unno

Authors
  1. N. Shiragami
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  2. H. Unno
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Shiragami, N., Unno, H. Effect of shear stress on activity of cellular enzyme in animal cell. Bioprocess Engineering 10, 43–45 (1994). https://doi.org/10.1007/BF00373534

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  • DOI: https://doi.org/10.1007/BF00373534

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