Abstract
We investigated the in vitro responses to bacterial superantigens of peripheral blood mononuclear cells taken from patients with psoriasis (one arthropathic, two guttate and four chronic plaque type). We also analysed the relationship between the magnitude of the responses of peripheral blood mononuclear cells to bacterial exotoxins and the number of circulating T cells bearing Vβ2 and Vβ3 regions. The proliferative response of peripheral blood mononuclear cells to Staphylococcal enterotoxin B and toxic shock syndrome toxin-1 was significantly higher in patients with active psoriasis than in normal subjects. An improvement in skin eruption was associated with a decrease in the lymphocyte response to one-half or one-third that of the active phase. There was no significant difference between patients with psoriasis and normal subjects in the percentage of Vβ2- and Vβ3-positive circulating T cells. The percentages of Vβ2-positive and Vβ3-positive cells were not correlated with the levels of responsiveness to toxic shock syndrome toxin-1 and to Staphylococcal enterotoxin B, respectively. These findings suggest that the magnitude of responses of peripheral blood mononuclear cells to bacterial toxins does not depend on the number of T cells reactive with the relevant superantigen, but depends on the extent of skin lesions in psoriasis.
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Baadsgaad O, Gupta AK, Taylor RS, Ellis CN, Voorheel JJ, Cooper KD (1989) Psoriasis epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells. J Invest Dermatol 92: 190–195
Baker BS, Bokth S, Powles A, Garioch JJ, Lewis H, Valdimsrsson H, Fry L (1993) Group A streptococcal antigen-specific T lymphocytes in guttate psoriatic lesions. Br J Dermatol 128: 493–499
Chin YH, Falanga V, Streilein JW, Sackstein R (1989) Lymphocyte proliferation of psoriatic endothelium: evidence for a tissue-specific receptor/ligand interaction. J Invest Dermatol 93: 82s-87s
Fredriksson T, Pettersson U (1978) Severe psoriasis: oral therapy with a new retinoid. Dermatologica 157: 238–244
Furue M, Katz S (1988) The effect of cyclosporine on epidermal cells: 1. Cyclosporine inhibits accessory cell functions of epidermal Langerhans cells in vitro. J Immunol 140: 4139–4143
Gottlieb AB, Lifshitz B, Fu SM, Staiano-Coico L, Wang CY, Carter DM (1986) Expression of HLA-DR molecules by keratinocytes, and presence of Langerhans cells in dermal infiltrates of active psoriatic plaques. J Exp Med 164: 1013–1028
Guilhou JJ, Meynadier J, Clot J, Charmasson E, Dardenne M, Brochier J (1976) Immunological aspects of psoriasis. II. Dissociated impairment of thymus-dependent lymphocytes. Br J Dermatol 95: 295–301
Herman A, Croteau G, Sekaly RP, Kappler J, Marrack P (1990) HLA-DR alleles differ in their ability to present Staphylococcal enterotoxins to T cells. J Exp Med 172: 709–717
Leung DYM, Walsh P, Giarno R, Norris DA (1993) A potential role for superantigens in the pathogenesis of psoriasis. J Invest Dermatol 100: 225–228
Lewis HM, Baker BS, Bokth S, Powles AV, Garioch JJ, Valdimarsson H, Fry L (1993) Restricted T-cell receptor Vβ gene usage in the skin of patients with guttate and chronic plaque psoriasis. Br J Dermatol 129: 514–520
McFadden J, Valdimarsson H, Fry L (1991) Cross-reactivity between streptococcal M surface antigen and human skin. Br J Dermatol 125: 443–447
Marples RR, Heaton CL, Klingman AL (1973) Staphylococcus aureus in psoriasis. Arch Dermatol 107: 568–570
Marrack P, Kappler J (1990) The staphylococcal enterotoxins and their relatives. Science 248: 705–711
Nickoloff BJ, Mitra RS, Green J, Zheng XG, Shimizu Y, Thompson C, Turka LA (1993) Accessory cell function of keratinocytes for superantigens: dependence on lymphocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction. J Immunol 150: 2148–22159
Norrlind R (1955) Significance of infections in the origin of psoriasis. Acta Rheumatol Scand 1: 135–144
Ramirez-Bosca A, Martinez-Ojeda L, Valcuende-Cavero F, Castells-Rodellas A (1988) A study of local immunity in psoriasis. Br J Dermatol 119: 587–595
Rosenberg EW, Belew PW (1982) Microbial factors in psoriasis. Arch Dermatol 118: 143–144
Rosenberg EW, Noah PW, Zanolli MD, Skinner RB, Bond MJ, Crutcher N (1986) Use of refampin with penicillin and erythromycin in the treatment of psoriasis. J Am Acad Dermatol 14: 761–764
Schmitt-Egenolf M, Boehncle WH, Ständer M, Eiermann TH, Sterry W (1993) Oligonucleotide typing reveals association of type I psoriasis with the HLA-DRB1*0701/2,-DQA1*0201,-DQB1*0303 extended haplotype. J Invest Dermatol 100: 749–752
Scholl PR, Deiz A, Karr R, Sekaly RP, Trowsdale J, Geha RS (1990) Effect of isotypes and allelic polymorphism on the binding of staphylococcal exotoxins to MHC class II molecules. J Immunol 144: 226–230
Tokura Y, Yagi J, O'Malley M, Lewis JM, Takigawa M, Edelson RL, Tigelaar RE (1994) Superantigenic staphylococcal enterotoxins induce T-cell proliferation in presence of Langerhans cells or class II-bearing keratinocytes and stimulate keratinocytes to produce T-cell-activating cytokines. J Invest Dermatol 102: 31–38
Whyte JH, Baughman RD (1964) Acute guttate psoriasis and streptococcal infection. Arch Dermatol 89: 350–356
Wilson AGMcT, Clark I, Heard SR, Munro DD, Kirby JDT (1993) Immunoblotting of streptococcal antigens in guttate psoriasis. Br J Dermatol 128: 151–158
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Yokote, R., Tokura, Y., Furukawa, F. et al. Susceptible responsiveness to bacterial superantigens in peripheral blood mononuclear cells from patients with psoriasis. Arch Dermatol Res 287, 443–447 (1995). https://doi.org/10.1007/BF00373426
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DOI: https://doi.org/10.1007/BF00373426