Abstract
The expression of two matrix metalloproteases (MMPs), 72 kDa gelatinase (MMP-2) and 92 kDa gelatinase (MMP-9), was studied in suction blister fluids and serum using a zymographic method. Both of the enzymes were detectable in blister fluid and serum, but their expression varied. The MMP-2 levels in serum were only about half of the levels in blister fluid, while the level of MMP-9 was about the same in both serum and blister fluid. The overall level of MMP-2 in serum was much lower than the level of MMP-9, which was possibly derived from inflammatory cells. In subjects aged from 20 to 86 years, no marked changes were seen in the serum or blister fluid gelatinases. Interestingly, body site affected the level of MMP-9. The lowest level was recorded in fluid from blisters on the lower leg, in which the level was only about 19% of the level in fluid from blisters on the abdomen. The levels of MMP-2 and MMP-9 were not decreased by pharmacological doses of systemic prednisone or isotretinoin, which indicates that systemic glucocorticoid or retinoid treatment does not affect the basal levels of MMP-2 and MMP-9.
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Anttinen H, Oikarinen A, Kivirikko KI (1977) Age-related changes in human skin collagen galactosyltransferase and collagen glucosyltransferase activities. Clin Chim Acta 76: 95–101
Bailly C, Dreze S, Asselineau D, Nusgens B, Lapiere CM, Darmon M (1990) Retinoic acid inhibits the production of collagenase by human epidermal keratinocytes. J Invest Dermatol 94: 47–51
Bauer EA, Kronberger A, Valle K-J, Jeffrey JJ, Eisen AZ (1985) Glucocorticoid modulation of collagenase expression in human fibroblast cultures: evidence for pre-translational inhibition. Biochim Biophys Acta 825: 227–235
Clark CD, Kobayashi DK, Welgus HG (1987) Regulation of the expression of tissue inhibitor of metalloproteinases and collagenase by retinoids and glucocorticoids in human fibroblasts. J Clin Invest 80: 1280–1288
Emonard H, Grimaud J-A (1990) Matrix metalloproteinases. A review. Cell Mol Biol 36: 131–153
Fessler LI, Duncan KG, Fessler JH, Salo T, Tryggvason K (1984) Characterization of the procollagen IV cleavage products produced by a specific tumor collagenase. J Biol Chem 259: 9783–9789
Hendrix MJC, Wood WR, Seftor EA, Lotan D, Nakajima M, Misiorowski RL, Seftor REB, Stetler-Stevenson WG, Bevacqua SS, Liotta LA, Sobel ME, Raz A, Lotan R (1990) Retinoic acid inhibition of human melanoma cell invasion through a reconstituted basement membrane and its relation to decreases in the expression of proteolytic enzymes and motility factor reseptor. Cancer Res 50: 4121–4130
Heussen C, Dowdle EB (1980) Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem 102: 196–202
Hurewitz AN, Zucker S, Mancuso P, Wu CL, Dimassimo B, Lysik RM, Moutsiakis D (1992) Human pleural effusions are rich in matrix metalloproteinases. Chest 102: 1808–1814
Kiistala U (1968) Suction blister device for separation of viable epidermis from dermis. J Invest Dermatol 50: 129–137
Nagase H, Ogata Y, Suzuki K, Enghild JJ, Salvesen G (1991) Substrate specificities and activation mechanisms of matrix metalloproteinases. Biochem Soc Trans 19: 715–718
O'Grady RL, Netherty A, Hunter N (1984) A fluorescent screening assay for collagenase using collagen labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone. Anal Biochem 140: 490–494
Oikarinen A, Salo T, Ala-Kokko L, Tryggvason K (1987) Dexamethasone modulates the metabolism of type IV collagen and fibronectin in human basement-membrane-forming fibrosarcoma (HT-1080) cells. Biochem J 245: 235–241
Oikarinen A, Kylmäniemi M, Autio-Harmainen H, Autio P, Salo T (1993) Demonstration of 72-kDa and 92-kDa forms of type IV collagenase in human skin: variable expression in various blistering diseases, induction during re-epithelialization, and decrease by topical glucocorticoids. J Invest Dermatol 101: 205–210
Salo T, Oikarinen J (1985) Regulation of type IV collagen degrading enzyme by cortisol during human skin fibroblast growth. Biochem Biophys Res Commun 130: 588–595
Seltzer JL, Eisen AZ, Bauer EA, Morris NP, Glanville RW, Burgeson RES (1989) Cleavage of type VII collagen by interstitial collagenase and type IV collagenase (gelatinase) derived from human skin. J Biol Chem 264: 3822–3826
Senior RM, Griffin GL, Fliszar CJ, Shapiro SD, Goldberg GI, Welgus HG (1991) Human 92- and 72-kilodalton type IV collagenases are elastases. J Biol Chem 266: 7870–7875
Stetler-Stevenson WG (1990) Type IV collagenases in tumor invasion and metastasis. Cancer Metastasis Rev 9: 289–303
Uitto J (1971) Collagen biosynthesis in human skin. A review with emphasis on scleroderma. Ann Clin Res 3: 250–258
Vartio T, Baumann M (1989) Human gelatinase/type IV procollagenase is a regular plasma component. FEBS Lett 255: 285–289
Vermeer BJ, Reman FC, van Gent CM (1979) The determination of lipids and proteins in suction blister fluid. J Invest Dermatol 73: 303–305
Welgus HG, Fliszar CJ, Seltzer JL, Schmid TM, Jeffrey JJ (1990) Differential susceptibility of type X collagen to cleavage by two mammalian interstitial collagenases and 72-kDa type IV collagenase. J Biol Chem 265: 13521–13527
Wilhelm SM, Collier IE, Marmer BL, Eisen AZ, Grant GA, Goldberg GI (1989) SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages. J Biol Chem 264: 17213–17221
Woodley DT, Kalebec T, Banes AS, Link W, Prunieras M, Liotta L (1986) Adult human keratinocytes migrating over nonviable dermal collagen produce collagenolytic enzymes that degrade type I and type IV collagen. J Invest Dermatol 86: 418–423
Zucker S, Lysik RM, Zarrabi MH, Stetler-Stevenson W, Liotta LA, Birkedal-Hansen H, Mann W, Furie M (1992) Type IV collagenase/gelatinase (MMP-2) is not increased in plasma of patients with cancer. Cancer Epidemiol Biomarkers Prev 1: 475–479
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Kylmäniemi, M., Autio, P. & Oikarinen, A. Influence of aging, localization, glucocorticoids and isotretinoin on matrix metalloproteases 2 (MMP-2) and 9 (MMP-9) in suction blister fluids. Arch Dermatol Res 287, 434–438 (1995). https://doi.org/10.1007/BF00373424
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DOI: https://doi.org/10.1007/BF00373424