Abstract
Decorin mRNA levels, the content of decorin and the synthesis of dermatan sulphate in skin fibroblasts from patients with systemic and localized scleroderma were investigated. Approximately a 2.2-fold increase in decorin mRNA levels, was found by Northern blot analysis in localized scleroderma, but no significant changes were found in systemic scleroderma. Decorin, as measured by an immunoblot assay, was increased 2.6-fold in fibroblast cultures from localized scleroderma but not in those from systemic scleroderma. In contrast, the synthesis of dermatan sulphate was similar in both conditions. These results indicate that altered decorin gene expression causes abnormal proteoglycan metabolism in localized scleroderma.
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Krieg T, Perlish JS, Mauch C, Fleischmajer R (1985) Collagen synthesis by scleroderma fibroblasts. Ann NY Acad Sci 460: 375–386
Scott JE (1988) Proteoglycan-fibrillar collagen interaction. Biochem J 252: 313–323
Hayes RL, Rodnan GP (1971) The ultrastucture of skin in progressive systemic sclerosis (scleroderma). Am J Path 63: 433–442
Vogel K, Paulsson M, Heinegård D (1984) Specific inhibition of type I and II collagen fibrillogenesis by the small proteoglycan of tendon. Biochem J 223: 587–597
Bidanset DJ, Guidry C, Rosenberg LC, Choi HU, Timpl R, Hook M (1992) Binding of proteoglycan decorin to collagen type VI. J Biol Chem 267: 5250–5256
Vogel KG, Koob TJ, Fisher LW (1987) Characterization and interaction of a fragment of the core protein of the small proteoglycan (PG II) from bovine tendon. Biochem Biophys Res Commun 148: 658–663
Yamaguchi Y, Ruoslahti E (1988) Expression of human proteoglycan in Chinese hamster ovary cells inhibits cell proliferation. Nature 336: 244–246
Yamaguchi Y, Mann DM, Ruoslahti E (1990) Negative regulation of transforming growth factor-β by the proteoglycan decorin. Nature 346: 281–284
Chomczynski P, Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159
Krusius T, Ruoslahti E (1986) Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA. Proc Natl Acad Sci USA 83: 7683–7687
Bernard MP, Chu M-L, Myers JC, Ramirez F, Eikenberry EF, Prockop DJ (1983) Nucleotide sequences of complementary deoxyribonucleic acids for the proα1 chain of human type I procollagen. Statistical evaluation of structures that are conserved during evolution. Biochemistry 22: 5213–5223
Johnstone B, Markopoulos M, Neame P, Caterson B (1993) Identification and characterization of glycanated and non-glycanated forms of biglycan and decorin in the human intervertebral disc. Biochem J 292: 661–666
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Sobue M, Nakashima N, Fukatsu T, Nagasaka T, Katoh T, Ogura T, Takeuchi J (1988) Production and characterization of monoclonal antibody to dermatan sulfate proteoglycan. J Histochem Cytochem 36: 479–485
Hata R, Nagai Y (1972) A rapid and micro method for separation of acidic glycosaminoglycans by two-dimensional electrophoresis. Anal Biochem 45: 462–468
Ninomiya Y, Hata R, Nagai Y (1980) Glycosaminoglycan synthesis by liver parenchymal cell clones in culture and its changes with transformation. Biochim Biophys Acta 675: 248–255
Schönherr E, Beavan LA, Hausser H, Kresse H, Culp LA (1993) Differences in decorin expression by papillary and reticular fibroblasts in vivo and in vitro. Biochem J 290: 893–899
Ninomiya Y, Hata R, Nagai Y, Tajima S, Nishikawa T, Hatano H (1982) Glycosaminoglycan metabolism by scleroderma fibroblasts in culture. Biomed Res 3: 70–82
Bashey RI, Millan A, Jimenez SA (1984) Increased biosynthesis of glycosaminoglycans by scleroderma fibroblasts in culture. Arthritis Rheum 27: 1040–1045
Fujii N, Nagai Y (1981) Isolation and characterization of a proteodermatan sulfate from calf skin. J Biochem 90: 1249–1258
Nakamura T, Matsunaga E, Shinkai H (1983) Isolation and some structural analyses of a proteodermatan sulfate from calf skin. Biochem J 213: 289–296
Damle SP, Cöster L, Gregory JD (1982) Proteodermatan sulfate isolated from pig skin. J Biol Chem 257: 5523–5527
Sampaio LO, Bayliss MT, Hardingham TE, Muir H (1988) Dermatan sulfate proteoglycan from human articular cartilage. Biochem J 254: 757–764
Fleischmajer R, Fisher LW, MacDonald D, Jacobs L, Perlish JS, Termine JD (1991) Decorin interacts with fibrillar collagen of embryonic and adult human skin. J Struct Biol 106: 82–90
Vuorio, Kähäri V-M, Black C, Vuorio E (1991) Expression of osteonectin, decorin, and transforming growth factor-β1 genes in fibroblasts cultured from patients with systemic sclerosis and morphea. J Rheumatol 18: 247–251
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Izumi, T., Tajima, S. & Nishikawa, T. Stimulated expression of decorin and the decorin gene in fibroblasts cultured from patients with localized scleroderma. Arch Dermatol Res 287, 417–420 (1995). https://doi.org/10.1007/BF00373421
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DOI: https://doi.org/10.1007/BF00373421