Skip to main content
SpringerLink
Log in

Transfection of murine LMTK− cells with purified HLA class I genes

VII. Association of allele- and locus-specific serological reactivities with respectively the first and second domains of the HLA-B7 molecule

  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The individual contributions of the first two external domains of the HLA-B7 heavy chain to the expression of allele-specific (B7) and locus-specific (B and C) antigenic determinants were investigated using hybrid class I genes. Hybrid genes were constructed in vitro by exon shuffling between the parent genes HLA-B7, HLA-Cw3, HLA-A3, and H-2K d, and their expression was monitored following transfection into mouse L cells. The results show that most allele-specific antigenic determinants are associated with the first external domain of the 137 heavy chain, whereas all the locus-specific antigenic determinants tested map to the second external domain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSA:

bovine serum albumin

FCS:

fetal calf serum

mAb:

monoclonal antibody

PBL:

peripheral blood lymphocytes

PBS:

phosphate-buffered saline

References

  • Arnold, B., Burgert, M. G., Hamann, U., Hämmerling, G., Kees, U., and Kvist, S.: Cytotoxic T cells recognize the two amino terminal domains of H-2K antigens in influenza A infected cells. Cell 38: 79–87, 1984

    Google Scholar 

  • Billing, R. and Lucero, K.: A monoclonal antibody against an epitope to HLA-B locus antigens. Hum. Immunol. 4: 351–357, 1982

    Google Scholar 

  • Ellis, S. A., Taylor, C., Hildreth, J. E. K., and McMichael, A. J.: A3 HLA class I specific monoclonal antibody that fails to bind to part of the HLA-A series. Hum. Immunol., in press, 1986

  • Engelhard, V. H., Yannelli, J. R., Evans, G. A., Walk, S. F., and Holterman, M. J.: Construction of novel class I histocompatibility antigen by interspecies exon shuffling. J. Immunol. 134: 4218–4225, 1985

    Google Scholar 

  • Ezquerra, A., Bragado, R., Strong, D. H., and Strominger, J. L.: Primary structure of papain-solubilized human histocompatibility antigen HLA-B27. Biochemistry 24: 1733–1741, 1985

    Google Scholar 

  • Ferrier, P., Fontecilla-Camps, J. C., Bucchini, D., Caillol, D. H., Jordan, B. R., and Lemonnier, F. A.: Altered structure of HLA class I heavy chains associated with mouse beta-2 microglobulin. Immunogenetics 21: 321–331, 1985

    Google Scholar 

  • Grosveld, F. G. Lund, T., Murray, E., Mellor, A., Dahl, A., and Flavell, R. A.: The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Res. 10: 6715–6732, 1982

    Google Scholar 

  • Jordan, B. R., Lemonnier, F. A., Caillol, D. H., and Trucy, J.: Transformation of murine LMTK− cells with purified HLA class I genes. III. Human HLA class I antigens coded by hybrid genes constructed in vitro indicate association of serologic reactivities with the first two domains of the molecule. Immunogenetics 18: 165–171, 1983

    Google Scholar 

  • Kandzia, J., Anderson, M.J.D., and Müller-Rüchholtz, W.: Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies. J. Cancer Res. Clin. Oncol. 101: 165–170, 1981

    Google Scholar 

  • Layet, C., Le Bouteiller, P. P., N'Guyen, C., Mercier, P., Rosa, F., Fellous, M., Caillol, D. H., Jordan, B. R., and Lemonnier, F. A.: Transformation of LMTK− cells with purified HLA class I gene. VI. Serological characterization of HLA-B7 and Aw24 molecules. Hum. Immunol. 11: 31–45, 1984

    Google Scholar 

  • Lopez de Castro, J. A., Strominger, J. L., Strong, D. M., and Orr, H. T.: Structure of cross-reactive human histocompatibility antigens HLA-A28 and HLA-A2: Possible implications for the generation of HLA polymorphism. Proc. Natl. Acad. Sci. U.S.A. 79: 3813–3817, 1982

    Google Scholar 

  • Lopez de Castro, J. A., Bragado, R., Strong, D. M., and Strominger, J. L.: Primary structure of papain-solubilized human histocompatibility antigen HLA-B40 (-BW60). An outline of alloantigenic determinants. Biochemistry 22: 3961–3969, 1983

    Google Scholar 

  • N'Guyen, C., Sodoyer, R., Trucy, J., Strachan, T., and Jordan, B. R.: The HLA-AW24 gene: Sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes. Immunogenetics 21: 479–489, 1985

    Google Scholar 

  • Orr, H. T., Lopez de Castro, J. A., Lancet, D., and Strominger, J. L.: Complete amino acid sequence of a papain-solubilized human histocompatibility antigen, HLA-B7. Sequence determination and search for homologies. Biochemistry 18: 5711–5720, 1979

    Google Scholar 

  • Ozato, K., Takahashi, H., Appell, A. E., Sears, D. W., Murre, C., Seidman, J. G., Kimura, S., and Tada, N.: Polymorphism of murine major histocompatibility class I antigen: Assignment of putative allodeterminants to distinct positions of the amino acid sequence within the first external domain of the antigen. J. Immunol. 134: 1749–1758, 1985

    Google Scholar 

  • Radka, S. F., Kostyu, D. D., and Amos, D. B.: A monoclonal antibody directed against the HLA-Bw6 epitope. J. Immunol. 128: 2804–2806, 1982

    Google Scholar 

  • Rebai, N. and Malissen, B.: Structural and genetic analyses of HLA class I molecules using monoclonal xenoantibodies. Tissue Antigens 22: 107–117, 1983

    Google Scholar 

  • Sher, B. T., Nairn, R., Coligan, J. E., and Hood, L. E.: DNA sequence of the mouse H-2D a transplantation antigen gene. Proc. Natl. Acad. Sci. U.S.A. 82: 1175–1179, 1985

    Google Scholar 

  • Sodoyer, R., Damotte, M., Delovitch, T. L., Trucy, J., Jordan, B. R., and Strachan, T.: Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3). EMBO J. 3: 879–886, 1984

    Google Scholar 

  • Strachan, T., Sodoyer, R., Damotte, M., and Jordan, B. R.: Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: Implications for the evolution of HLA genes. EMBO J. 3: 887–894, 1984

    Google Scholar 

  • Ways, J. P., Coppin, H. L., and Parham, P.: The complete primary structure of HLA-Bw58. J. Biol. Chem. 260: 11924–11933, 1985

    Google Scholar 

  • Wigler, M., Sweet, R., Sim, G. K., Wold, B., Pellicer, A., Lacy, E., Maniatis, T., Silverstein, S., and Axel, R.: Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell 16: 777–785, 1979

    Google Scholar 

  • Yang, S. Y., Morishima, Y., Collins, N. H., Alton, T., Pollack, M. S., Yunis, E. J., and Dupont, B.: Comparison of one-dimensional IEF patterns for serologically detectable HLA-A and B allotypes. Immunogenetics 19: 217–231, 1984

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288, Marseille Cédex 9, France

    Régis Sodoyer, Brigitte Kahn-Perles, Joséphine Sire, Marie-José Santoni, Corine Layet, Pierre Ferrier, Bertrand R. Jordan & Francois A. Lemonnier

  2. Department of Medical Genetics, University of Manchester, M13 OJH, Manchester, UK

    Torn Strachan

Authors
  1. Régis Sodoyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brigitte Kahn-Perles
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Torn Strachan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joséphine Sire
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marie-José Santoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Corine Layet
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Pierre Ferrier
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Bertrand R. Jordan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Francois A. Lemonnier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sodoyer, R., Kahn-Perles, B., Strachan, T. et al. Transfection of murine LMTK− cells with purified HLA class I genes. Immunogenetics 23, 246–251 (1986). https://doi.org/10.1007/BF00373019

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00373019

Keywords

Search

Navigation