Abstract
A mathematical model for the design of bubble-columns for growth of shear-sensitive insect cells is presented. The model is based on two assumptions. First, the loss of cell viability as a result of aeration is a first-order process. Second, a hypothetical volume X, in which all viable cells are killed, is associated with each air bubble during its lifetime. The model merely consists of an equation for k d, the first-order death-rate constant, and A min, the minimum specific surface area of the air bubbles to supply sufficient oxygen. In addition to X, the equation for k d contains the air flow F, the air-bubble diameter d b, the diameter D and the height H of the bubble column. This equation has been experimentally validated. Comparison of the equations for k d and A min shows that especially H is the key parameter to manipulate in bubble-column design in order to meet the demands set by A min and k d<kg, the first-order growth-rate constant. It is concluded that net growth of cells is enhanced as size and height of the bubble column increase.
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References
Cherry, R. S.; Papoutsakis, E. T.: Hydrodynamic effects on cells in agitated tissue culture reactors. Bioprocess Eng. 1 (1986) 29–41
Croughan, M. S.; Hamel, J. F.; Wang, D. I. C: Hydrodynamic effects on animal cells grown in microcarrier cultures. Biotechnol. Bioeng. 29 (1987) 130–141
Emery, A. N.; Lavery, M.; Williams, B.; Handa, A.: Large-scale hybridoma culture. In: Webb, C.; Mavituna, F. (Eds.): Plant and animal cell cultures: Process possibilities, pp. 137–146. Chichester: Ellis Horwood 1987.
Handa, A.; Emery, A. N.; Spier, R. E.: On the evaluation of gas-liquid interfacial effects on hybridoma viability in bubble column bioreactors. Presented at 7th ESACT meeting, Baden, Austria. To be published in Develop. Biol. Stand.
Tramper, J.; Joustra, D.; Vlak, J. M.: Bioreactor design for growth of shear-sensitive insect cells. In: Webb, C.; Mavituna, F. (Eds.): Plant and animal cell cultures: Process possibilities, pp. 125–136. Chichester: Ellis Horwood 1987
Tramper, J.; Williams, J. B.; Joustra, D.; Vlak, J. M.: Shear sensitivity of insect cells in suspension. Enzyme Microb. Technol. 8 (1986) 33–36
Tramper, J.; Vlak, J. M.: Some engineering and economic aspects of continuous cultivation of insect cells for the production of baculoviruses. Ann. N. Y. Acad. Sci. 469 (1986) 279–288
Vaughn, J. L.; Goodwin, R. H.; Tompkins, G. J.; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13 (1977) 231–217
Hink, W. F.: Established insect cell line from the cabbage looper, Trichoplusia ni. Nature 226 (1970) 466–467
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Tramper, J., Smit, D., Straatman, J. et al. Bubble-column design for growth of fragile insect cells. Bioprocess Engineering 3, 37–41 (1988). https://doi.org/10.1007/BF00372858
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DOI: https://doi.org/10.1007/BF00372858