Skip to main content
SpringerLink
Log in

Experience in using an ethanol sensor to control molasses feed-rates in baker's yeast production

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

An ethanol sensor has been tested for feed-rate control of baker's yeast prouction. The yeast was grown on molasses in an 8 dm3 fed-batch reactor up to a cell concentration of 60–70 kg/m3. Studies were made on three levels: reliability of the sensor system, characterisation of the control problem, and evaluation of ethanol-controlled cultivations in terms of yield and production rate. Arguments are given for the conceptual advantages of ethanol control compared to other methods of substrate control. It is also shown that ethanol control allows for a simple regulator structure. In fact, a PID regulator, with constant parameters, was used around an exponential dosage scheme. Tuning of the regulator parameters was performed by using simulation on a simplified model of the process. Several cultivations have been carried out. Results from four comparable cultivations are given in detail, and the experience from many others is summarized.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. De Deken, R. H.: The Crabtree effect. A regulatory system in yeast. J. Gen. Microbiol. 44 (1966) 149

    Google Scholar 

  2. Sonnleitner, B.; Käppeli, O.: Growth of Saccharomyces cerevisiae is controlled by its limited respiratory capacity: formulation and verification of a hypothesis. Biotechnol. Bioeng. 28 (1986) 927–937

    Google Scholar 

  3. Aiba, S.; Nagai, S.; Nishizawa, Y.: Fed-batch cultures of S. cerevisiae: a perspective of computer control to enhance the productivity in baker's yeast cultivation. Biotechnol. Bioeng. 18 (1976) 1001–1016

    Google Scholar 

  4. Wang, H.; Cooney, C.; Wang, D.: Computer aided baker's yeast fermentations. Biotechnol. Bioeng. 19 (1977) 69–86

    Google Scholar 

  5. Dekkers, R. M.; Voetter, M: Adaptive control of a fed-batch baker's yeast fermentation. In: Johnson, A. (Ed): Modelling and control of biotechnological processes — 1st IFAC symposium, preprints, pp. 73–80. Oxford: Pergamon Press 1985

    Google Scholar 

  6. Montgomery, P. A.; Williams, D.; Swanick, B. H.: Control of a fermentation process by an on-line adaptive technique. In: Johnson, A. (Ed): Modelling and control of biotechnological processes — 1st IFAC symposium, preprints. pp. 81–89. Oxford: Pergamon Press 1985

    Google Scholar 

  7. Verbruggen, H. B.; Eelderink, G.; van den Broecke, P. M.: Multiloop controlled fed-batch fermentation process using a selftuning controller. In: Johnson, A. (Ed): Modelling and control of biotechnological processes — 1st IFAC symposium, preprints. pp. 91–96. Oxford: Pergamon Press 1985

    Google Scholar 

  8. Reuss, M.; Brammer, U.: Influence of substrate distribution on productivities in computer controlled baker's yeast production. In: Johnson, A. (Ed): Modelling and control of biotechnological processes — 1st IFAC symposium, preprints. pp. 119–124. Oxford: Pergamon Press 1985

    Google Scholar 

  9. Yamané, T.; Matsuda, M.; Sada, E.: I. Application of porous teflon tubing method to automatic fed-batch culture of microorganisms. II. Automatic constant-value control of fed substrate (ethanol) concentration in semibatch culture of yeast. Biotechnol. Bioeng. 23 (1981) 2509–2524

    Google Scholar 

  10. Dairaku, K.; Yamasaki, Y.; Kuki, K.; Shioya, S.; Takamatsu, T.: Maximum production in a baker's yeast fed-batch culture by tubing method. Biotechnol. Bioeng. 23 (1981) 2069–2081

    Google Scholar 

  11. Dairaku, K.; Izumoto, E.; Morikawa, H.; Shioya, S.; Takamatsu, T.: An advanced micro-computer coupled control system in a baker's yeast fed-batch culture using a tubing method. J. Ferment. Technol. 61 (1983) 189–196

    Google Scholar 

  12. Axelsson, J. P.; Hagander, P.; Holst, O.; Mandenius, C. F.; Mattiasson, B.: Control of baker's yeast production based on ethanol measurement. In: Third European congress of biotechnology, Munich, 10–14 sept. 1984, preprint Vol. II (1984) 629–634

    Google Scholar 

  13. Johnson, M. J.; Borkowski, J.; Engblom, C.: Steam sterilizable probes for dissolved oxygen measurement. Biotechnol. Bioeng. 6 (1984) 457–468

    Google Scholar 

  14. Kobayashi, T.; Yano, T.; Mori, H.; Shimizu, S.: Cultivations of microorganisms with DO-stat and a silicone tubing sensor. Biotechnol. Bioeng. Symp. 9 (1979) 73–83

    Google Scholar 

  15. Mandenius, C. F.; Mattiasson, B.: Improved membrane gas sensor-systems for on-line analysis of ethanol and other volatile organic compounds in fermentation media. Eur. J. Appl. Microbiol. Biotechnol. 18 (1983) 197–200

    Google Scholar 

  16. Mandenius, C. F.; Holst, O.; Mattiasson, B.: Monitoring of ethanol in production of baker's yeast using an improvement membrane gas sensor. Acta Chemica Scandinavica, B 37 (1983) 746–748

    Google Scholar 

  17. Elmqvist, H.; Mattson, S. E.: Implementation of real-time facilities in PASCAL. In: SOCOCO 82, 3rd IFAC/IFIP Symposium on solftware for computer control, Madrid, 5–8 October 1982

  18. Fukuda, H.; Shiotani, T.; Okada, W.; Morikawa, H.: A mathematical model of the growth of baker's yeast subject to product inhibition. J. Ferment. Technol. 56 (1978) 361–368

    Google Scholar 

  19. Maiorella, B. L.; Blanch, H. W.; Wilke, R. C.: By-product inhibition effects on ethanolic fermentation by S. cerevisiae. Biotechnol. Bioeng. 25 (1983) 103–121

    Google Scholar 

  20. Maiorella, B. L.; Blanch, H. W.; Wilke, R. C: Feed component inhibition in ethanolic fermentation by S. cerevisiae. Biotechnol. Bioeng. 26 (1984) 1155–1166

    Google Scholar 

  21. Pons, M.-N.; Rajab, A.; Engasser, J. M.: Influence of acetate on growth kinetics and production control of S. cerevisiae. Appl. Microbiol. Biotechnol. 24 (1986) 193–198

    Google Scholar 

  22. Wu, W. T.; Chen, K. C.; Chiou, H. W.: On-line optimal control for fed-batch culture of baker's yeast production. Biotechnol. Bioeng. 27 (1985) 755–760

    Google Scholar 

  23. Dairaku, K.; Izumoto, E.; Morikawa, H.; Shioya, S.; Takamatsu, T.: Simulation and quality control of baker's yeast fedbatch culture using population dynamics. In: processing of Chemcomp 82, Antwerpen, Belgium, 1982.

  24. Shioya, S.; Shimizu, H.; Ogata, M.; Takamatsu, T.: Simulation and experimental studies of the profile control of the specific growth rate in a fed-batch culture. In: Johnson, A. (Ed): Modelling and control of biotechnological processes — 1st IFAC symposium, preprints. pp. 49–54. Oxford: Pergamon Press 1985

    Google Scholar 

  25. Bastin, G.; Dochain, D.: On-line estimation of microbial specific growth rates. Automatica. 22 (1986) 705–709

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Automatic Control, Lund Institute of Technology, P.O. Box 118, S-22100, Lund, Sweden

    J. P. Axelsson & P. Hagander

  2. Department of Pure and Applied Biochemistry, Chemical Center, Lund Institute of Technology, P.O. Box 118, S-22100, Lund, Sweden

    C. F. Mandenius

  3. Department of Biotechnology, Chemical Center, Lund Institute of Technology, P.O. Box 118, S-22100, Lund, Sweden

    O. Holst & B. Mattiasson

Authors
  1. J. P. Axelsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. F. Mandenius
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Holst
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Hagander
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Mattiasson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Axelsson, J.P., Mandenius, C.F., Holst, O. et al. Experience in using an ethanol sensor to control molasses feed-rates in baker's yeast production. Bioprocess Engineering 3, 1–9 (1988). https://doi.org/10.1007/BF00372854

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00372854

Keywords

Search

Navigation