Skip to main content

Effects of cepharanthine and minoxidil on proliferation, differentiation and keratinization of cultured cells from the murine hair apparatus

Archives of Dermatological Research volume 284, pages 290–296 (1992)Cite this article

Summary

The effects of cepharanthine and minoxidil on proliferation, differentiation and keratinization of cultured cells from the murine hair apparatus were examined electron microscopically. Both cepharanthine and minoxidil stimulated cell proliferation and delayed initiation of differentiation and keratinization of the cultured cells. On day 6, most control cells (87%) cultured in a 0.03 mM calcium medium without cepharanthine and minoxidil were differentiated into several subpopulations corresponding to those of in vivo cell layers of the hair apparatus, while most of the cells cultured with cepharanthine (71%) or minoxidil (70%) were still immature. On day 13, the number of degenerated cells increased (63%) in the control culture, whereas in the culture treated with cepharanthine or minoxidil, cell degeneration scarcely occurred (5% and 8%, respectively). Differentiated cells having tonofilaments were often observed in the cepharanthine- and minoxidil-treated cultures (76% and 72%, respectively). Elevation of extracellular calcium up to 1.0 mM induced keratinization (34%) in the control culture on day 6, while no keratinized cells were observed in the cepharanthine- or minoxidil-treated culture. On day 13 keratinization similarly occurred in the cultures with cepharanthine (30%) or minoxidil (48%). These results show that both cepharanthine and minoxidil may directly influence proliferation, differentiation and keratinization of cultured cells from the hair apparatus.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. Asano M, Ohkubo C, Sasaki A, Sawanobori K, Nagano H (1987) Vasodilator effects of cepharanthine, a biscoclaurine alkaloid, on cutaneous microcirculation in the rabbit. J Ethnopharmacol 20: 107–120

    Google Scholar 

  2. Buhl AE, Waldon DJ, Kawabe TT, Holland JM (1989) Minoxidil stimulates mouse vibrissae follicles in organ culture. J Invest Dermatol 92: 315–320

    Google Scholar 

  3. Dargie HJ, Dollery CT, Daniel J (1977) Minoxidil in resistant hypertension. Lancet II: 515–518

    Google Scholar 

  4. Devine BL, Fife R, Trust PM (1977) Minoxidil for severe hypertension after failure of other hypertensive drugs. BMJ 2: 667–669

    Google Scholar 

  5. Edashige K, Utsumi T, Utsumi K (1991) Inhibition of 12-O-tetradecanoyl phorbol-13-acetate promoted tumorigenesis by cepharanthine, a biscoclaurine alkaloid, in relation to the inhibitory effect on protein kinase C. Biochem Pharmacol 41: 71–78

    Google Scholar 

  6. Hashimoto K, Shibazaki S (1976) Ultrastructural study on differentiation and function of hair. In: Kobori T, Montagna W (eds) Biology and disease of the hair. University of Tokyo, Tokyo, pp 23–57

    Google Scholar 

  7. Headington JT, Novak E (1984) Clinical and histological studies of male pattern baldness treated with topical minoxidil. Curr Ther Res Clin Exp 36: 1098–1106

    Google Scholar 

  8. Kasajima T, Yamakawa M, Maeda K, Matsuda D, Dobashi M, Imai Y (1973) An experimental study on effect of cepharanthine to leukopenia induced by administration of anticancer drugs. Jpn J Cancer Chemother 10: 1180–1196

    Google Scholar 

  9. Lullmann H, Wehling M (1979) The binding of drugs to different polar lipids in vitro. Biochem Pharmacol 28: 3409–3415

    Google Scholar 

  10. Lullmann H, Lullmann RR, Wassermann O (1978) Lipidosis induced by amphiphilic cationic drugs. Biochem Pharmacol 27: 1103–1108

    Google Scholar 

  11. Nagashima H, Kuribayashi T (1968) Treatment of alopecia areata with cepharanthine. Dermatol Urol 30: 692–697

    Google Scholar 

  12. Sugiyama K, Sasaki J, Utsumi K, Miyahara M. (1976) Inhibition by cepharanthine of histamine release from rat peritoneal mast cells. Allergy 25: 383–389

    Google Scholar 

  13. Takeuchi T, Sato S (1985) Effect of cepharanthine on cell-type conversion in B16 mouse melanoma cells. J Dermatol 12: 138–142

    Google Scholar 

  14. Tanigaki N, Nakamura S, Kanehisa H, Masamoto Y, Kitano Y (1989) Methods for optimized cultivation of hair cells from C3H mice. Jpn J Dermatol 99: 1145–1152

    Google Scholar 

  15. Tanigaki N, Ando H, Ito M, Hashimoto A, Kitano Y (1990) Electron microscopic study of cultured cells from the murine hair tissues: cell growth and differentiation. Arch Dermatol Res 282: 402–407

    Google Scholar 

  16. Tanigaki N, Ito M, Ando H, Kitano Y, Hashimoto Y, Masamoto Y (1990) Calcium regulation of growth, differentiation and keratinization of cultured cells from murine hair and hair follicles: an ultrastructural study. Acta Med Biol 38: 187–192

    Google Scholar 

  17. Tsuzaki Y, Akiyama N, Kanemoto K, Ishizaki M, Nemoto M, Kikuchi Y (1981) Considerations on therapeutic experiences in snake bite, from 58 cases during 13 years. Surgery 43: 1023–1027

    Google Scholar 

  18. Uno H, Cappas A, Schlagel C (1985) Cyclic dynamics of hair follicles and the effect of minoxidil on the bald scalps of stumptailed macaques. Am J Dermatopathol 7: 283–297

    Google Scholar 

  19. Wester RC, Maibach HI, Guy RH, Novak E (1984) Minoxidil stimulates cutaneous blood flow in human balding scalps: pharmacodynamics measured by laser doppler velocimetry and photopulse plethysmography. J Invest Dermatol 82: 515–517

    Google Scholar 

Download references

Author information

Affiliations

  1. Fundamental Research Laboratory, R & D Division, Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan

    N. Tanigaki-Obana

  2. Department of Dermatology, Niigata University of Medicine, Niigata, Japan

    M. Ito

Authors
  1. N. Tanigaki-Obana
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tanigaki-Obana, N., Ito, M. Effects of cepharanthine and minoxidil on proliferation, differentiation and keratinization of cultured cells from the murine hair apparatus. Arch Dermatol Res 284, 290–296 (1992). https://doi.org/10.1007/BF00372583

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00372583

Key words

  • Cepharanthine
  • Minoxidil
  • Culture
  • Hair cells
  • Electron microscopy