Abstract
An analysis is presented of time lapse motion picture films showing the effects of DL-p-fluorophenylalanine (PFPA) on chromosome movement in cultured human amnion cells. The data indicate that PFPA has the unusual effect of slowing the rate at which chromosomes move in anaphases which occur after the beginning of treatment. Irrespective of treatment, cytokinesis begins when chromsome movement has progressed to a relatively fixed point. Thus, a slowdown in the rate of chromosome movement leads to a delay in initiation of cytokinesis. Cytokinesis, once it begins, is not affected by treatment with the analog.
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Behnke, O., Forer, A., Emmersen, J.: Actin in sperm tails and meiotic spindles. Nature (Lond.) 234, 408–410 (1971)
Gawadi, N.: Actin in the mitotic spindle. Nature (Lond.) 234, 410 (1971)
Horowitz, N. H., Fling, M., Feldman, H. M., Pall, M. L., Froehner, S. C.: Derepression of tyrosinase synthesis in Neurospora by amino acid analogs. Develop. Biol. 21, 147–156 (1970)
Inoué, S.: Organization and function of the mitotic spindle. In: Primitive motile systems in cell biology (R. D. Allen and N. Kamiya, eds.), p. 549–594. New York: Academic Press 1964
Inoué, S., Sato, H.: Cell motility by labile association of molecules. J. gen. Physiol., Suppl. 50, 259–288 (1967)
Jeantet, Cl., Gomes, R. A., Monier, R.: Effet de la p-fluorophenylalanine sur la formation des ribosomes chez Escherichia coli. Bull. Soc. Chem. Biol. 50, 473–489 (1968)
Kang, S., Rocky, P., Markovitz, A.: Derepression of β-galactosidase synthesis in Escherichia coli K-12 by PFPA. J. Bact. 96, 139–145 (1968)
Leick, V.: Effect of actinomycin D and DL-p-fluorophenylalanine on ribosome formation in Tetrahymena pyriformis. Europ. J. Bioch. 8, 215–220 (1969)
Nicklas, R. B.: Mitosis. Advanc. Cell Biol. 2, 225–297 (1971)
Rappaport, R.: Cytokinesis in animal cells. Int. Rev. Cytol. 31, 169–213 (1971)
Ris, H.: The anaphase movement of chromosomes in the spermatocytes of the grasshopper. Biol. Bull. 96, 90–106 (1949)
Sisken, J. E., Iwasaki, T.: The effects of some amino acid analogs on mitosis and the cell cycle. Exp. Cell Res. 55, 161–167 (1969)
Sisken, J. E., Morasca, L., Kibby, S.: Effects of temperature on the mitotic cycle of mammalian cells in culture. Exp. Cell Res. 39, 103–116 (1965)
Sisken, J. E., Stevens, E. E., Donnelly, M.: Effects of p-DL-fluorophenylalanine on mitosis and the mitotic spindle. Exp. Cell Res. 73, 25–32 (1972)
Sisken, J. E., Wilkes, E.: The time of synthesis and the conservation of mitosisrelated proteins in cultures human amnion cells. J. Cell Biol. 34, 97–110 (1967)
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Sisken, J.E. The effects of p-DL-fluorophenylalanine on chromosome movement and cytokinesis of human amnion cells in culture. Chromosoma 44, 91–98 (1973). https://doi.org/10.1007/BF00372575
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DOI: https://doi.org/10.1007/BF00372575