Abstract
Effects of amino acids on puffing in salivary gland chromosomes of Chironomus thummi are described. All twenty naturally occurring amino acids except tyrosine were tested. Of these, only tryptophan and phenylalanine proved effective. Both induced puffs at the same seven loci: Ia3/4, Ie3, If4, Ig1, Ig2, IIId1 and IIId1/2. The induction is energy dependent and requires concomitant RNA synthesis. Amino acid induced puffs incorporate 3H-uridine into acid insoluble material. At least one monovalent plus one divalent cation has to be present along with the amino acid in the incubation medium for puff induction.
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Gopalan, H.N.B. Amino acid induced changes of puffing patterns in Chironomus salivary gland chromosomes. Chromosoma 44, 25–32 (1973). https://doi.org/10.1007/BF00372571
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DOI: https://doi.org/10.1007/BF00372571