Abstract
Interleukin-1 expression is reported to be modified under a number of cell conditions including physiological stress, injury and activation. We report the effects of the physiological stresses cold and heat shock on IL-1 levels in keratinocytes. Having observed that normal human skin obtained from plastic surgery, usually stored at 4‡C for a few hours, highly expressed HSP72, a constant feature of stressed human keratinocytes, we wondered whether this induction could be linked to a cold shock and to modification of IL-1 levels in keratinocytes. Cultured keratinocytes were incubated at 4, 37, 40 and 43‡C for 1.5, 4, 8 and 16 h in a defined medium. HSP72 expression was studied by immunohistochemistry and immunoblot and IL-1 was quantified using specific and sensitive radio-immunoassay. Our findings showed that intracellular IL-1α and IL-1Β levels are not significantly modified by thermal shock. HSP72 is only induced after cell exposure to 43‡C and is not a cold-shock protein. These results demonstrate that thermal stress is not an inductive signal for IL-1 modification in keratinocytes.
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Gatto, H., Viac, J., Charveron, M. et al. Effects of thermal shocks on interleukin-1 levels and heat shock protein 72 (HSP72) expression in normal human keratinocytes. Arch Dermatol Res 284, 414–417 (1992). https://doi.org/10.1007/BF00372072
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DOI: https://doi.org/10.1007/BF00372072