Abstract
The hypothesis of indirect mitotic nondisjunction was tested in plant and mammalian cells. This hypothesis states that micronuclei derived from lagging chromosomes or chromatids are able to perform DNA synthesis and undergo mitotic condensation synchronously with main nuclei. Hence, as chromosomes, they can be moved to spindle poles together with the chromosomes of the main nuclei during mitosis. In that way chromosomes “lost” as micro-nuclei can be reincorporated in the main nuclei. In order to test this, both Vicia faba meristematic cells and cells of a Chinese hamster line (Cl-1) were treated with low doses of colchicine. Mitotic anomalies, micronuclei and cells with a polyploid or aneuploid karyotype were scored at different fixation times. A detailed analysis was performed on single chromosome misdistributions, as well as on micronuclei and cells with aneuploid karyotypes derived from single chromosome misdistributions. Indirect mitotic nondisjunction was shown to play a primary role in the origin of aneuploid karyotypes in Vicia faba, but not in Cl-1 cells.
Similar content being viewed by others
References
Danford N (1984) Measurement of levels of aneuploidy in mammalian cells using a modified hypotonic treatment. Mutat Res 139:127–132
Das NK (1962) Synthetic capacities of chromosome fragments correlated with their ability to maintain nucleolar material. J Cell Biol 5:121–130
Degrassi F, Rizzoni M (1982) Micronucleus test in Vicia faba root tips to detect mutagen damage in fresh-water pollution. Mutat Res 97:19–33
Deysson B (1968) Antimitotic substances. Int Rev Cytol 24:99–148
Ford JH (1984) Spindle microtubular dysfunction in mothers of Down Syndrome children. Hum Genet 68:295–298
Ford JH (1985) A model for the mechanism of aneuploidy involving chromosome displacement. In: Dellarco VL, Voytek PE, Hollaender A (eds) Aneuploidy, ethiology and mechanisms. Plenum Press, NY, pp 291–295
Ford JH, Lester P (1982) Factors affecting the displacement of human chromosomes from the metaphase plate. Cytogenet Cell Genet 33:327–332
Ford JH, Roberts CG (1983) Displacement of chromosomes in mitosis: a technique for assessing differential chromosome error. Cytogenet Cell Genet 36:537–541
Frackowiak S, Labidi B, Hernandez-Verdun D, Bouteille M (1986) Preservation of chromosome integrity during micronucleation induced by colchicine in PtK-1 cells. Chromosoma 94:468–474
Ghosh S, Paweletz N (1984) Synchronous DNA synthesis and mitosis in multinucleate cells with one chromosome in each nucleus. Chromosoma 89:197–200
Griffiths TD, Carpenter JG (1979) Premature chromosome condensation following X-irradiation of mammalian cells: expression time and dose response. Radiat Res 79:187–202
Gustavino B, Vitagliano E, Scottili A, Rizzoni M (1987) A comparison between short term evolution of micronuclei induced by X rays and colchicine in root tips of Vivia faba. Mutat Res 192:109–121
Hsu TC, Satya-Prakash KL (1985) Aneuploidy induction by mitotic arrestants in animal cell systems: possible mechanisms. In: Dellarco VL, Voytek PE, Hollaender A (eds) Aneuploidy, ethiology and mechanisms. Plenum Press, NY, pp 279–289
Hsu TC, Liang JL, Shirley LR (1983) Aneuploidy induction by mitotic arrestants: effects of diazepam on diploid Chinese hamster cells. Mutat Res 122:201–209
Ikeuchi T (1973) Chromosome pulverization in Chinese hamster multimicleate cells induced by bleomycin and cytochalasin-B. Jpn J Genet 48:391–405
Kato H, Sandberg AA (1967) Chromosome pulverization in human binucleate cells following colcemide treatment. J Cell Biol 34:35–45
Kato H, Sandberg AA (1968) Chromosome pulverization in human cells with micronuclei. J Natl Cancer Inst 40:167–179
Kihlman BA (1975) Root tips of Vicia faba for the study of the induction of chromosomal aberrations. Mutat Res 31:401–412
McLeish J (1954) The consequences of localized chromosome breakage. Heredity 8:385–407
Murata M, Vig BK (1980) Sequence of centromere separation: analysis of mitotic chromosomes in a reconstructed karyotype of Vicia faba. L Biol Zentralbl 99:686–693
Obe G, Beek B (1975) The human leukocyte test system. VII. Further investigations concerning micronucleus-derived premature chromosome condensation. Humangenetik 30:143–154
Obe G, Beek B (1982) Premature chromosome condensation in micronuclei. In: Rao PT, Johnson RT, Sperling K (eds) Premature chromosome condensation. Application in basic, clinical and mutation research. Academic Press, NY, pp 113–130
Östergren G, Levan A (1943) The connection between c-mitotic activity and water solubility in some monocyclic compounds. Hereditas 29:496–498
Pincu M, Callisen H, Norman A (1985) DNA content of micronuclei in human lymphocytes. Int J Radiat Biol 47:423–432
Rainaldi G, Flori L, Colella CL, Mariani T, Piras A, Simi S, Simili M (1987) Analysis by BrudR-labelling technique of induced aneuploidy in mammalian cells in culture. Mutat Res 177:255–260
Raman R (1980) Asynchrony in proliferation of sister nuclei in colcemid-induced polykaryons of muntjak lymphocytes. Indian J Exp Biol 18:115–119
Satya-Prakash KL, Hsu TC, Wheeler WJ (1984) Metaphase arrest, anaphase recovery and aneuploidy induction in cultured Chinese hamster cells following exposure to mitotic arrestants. Anticancer Res 4:351–356
Scott D, Evans HJ (1964) Influence of the nucleolus on DNA synthesis and mitosis in Vicia faba. Exp Cell Res 36:145–159
Stubblefield E (1964) DNA synthesis and chromosomal morphology of Chinese hamster cells in media containing N-deacetyl-N-methylcolchcine (Colcemid). Symp Int Soc Cell Biol. 3:223–248
Vig BK (1981) Sequence of centromere separation: analysis of mitotic chromosomes in man. Hum Genet 57:247–252
Vig BK (1984) Sequence of centromere separation: another mechanism for the origin of non disjunction. Hum Genet 66:239–243
Vig BK, Miltenburger MG (1976) Sequence of centromere separation of mitotic chromosomes in Chinese hamster. Chromosoma 5:75–80
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rizzoni, M., Tanzarella, C., Gustavino, B. et al. Indirect mitotic nondisjunction in Vicia faba and Chinese hamster cells. Chromosoma 97, 339–346 (1989). https://doi.org/10.1007/BF00371976
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00371976