Summary
The capability to distinguish eccrine gland cells of the ductal compartment from the secretory portion on the molecular level, as well as from epidermal keratinocytes and other skin cells, is of importance for the study of eccrine differentiation and function. Furthermore, the assessment of differences between the cell systems is useful for the characterization of benign and malignant neoplasms derived from eccrine glands. In the present study, we analysed the expression of four selected epithelial cell surface glycoproteins (gp 80, gp 38, gp 115 and gp 200) in eccrine glands using specific monoclonal antibodies. We found that the glycoproteins are differentially expressed in the ductal compartments and in the secretory portion of the glands, as well as in normal epidermis and other skin cells. This permitted the assignment of specific phenotypes to cells of the ductal compartment and to those of the secretory portion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aiba S, Masuko T, Hosokawa M, Hashimoto Y (1983) Monoclonal antibodies to various morphological components of human skin. J Invest Dermatol 81:498–502
Argenyi Z, Balogh K, Goeken JA (1988) Immunohistochemical characterization of chondroid syringomas. Am J Clin Pathol 90:662–669
Audo I, Tamaki K (1985) HNK-I antibody reacts with peripheral nerves and sweat glands in the skin. Br J Dermatol 113:175–178
Hashimoto K, Gross BG, Lever WF (1966) The ultrastructure of human embryo skin. II. The formation of the intradermal portion of the eccrine sweat duct and of the secretory segment during the first half of embryonic life. J Invest Dermatol 46:513–529
Kanitakis J, Schmitt D, Bernard A, Boumsell L, Thivolet J (1983) Anti-D47: a monoclonal antibody reacting with the secretory cells of human eccrine sweat glands. Br J Dermatol 109:509–513
Kanitakis J, Zambruno G, Viac J, Panzini H, Thivolet J (1987) Expression of neural-tissue markers (S-100 protein and Leu-7 antigen) by sweat gland tumors of the skin. J Am Acad Dermatol 17:187–191
Klein CE, Cordon-Cardo C, Soehnchen R, Cote RJ, Oettgen MF, Eisinger M, Old LJ (1987) Changes in cell surface glycoprotein expression during differentiation of human keratinocytes. J Invest Dermatol 89:500–506
Klein CE, Hartmann B, Schön MP, Weber L, Alberti S (1990) Expression of a 38 kD cell surface glycoprotein in transformed human keratinocyte cell lines, basal cell carcinomas, and epithelial germs. J Invest Dermatol 95:74–82
Ma ASP, Sun TT (1986) Differentiation-dependent changes in the solubility of a 195 kD protein in human epidermal keratinocytes. J Cell Biol 103:41–48
Maiorana A, Nigrisoli E, Papotti M (1986) Immunohistochemical markers of sweat gland tumors. J Cutan Pathol 13:187–196
Mattes MJ, Cairncross JG, Old LJ, Lloyd KO (1983) Monoclonal antibodies to three widely distributed human cell surface antigens. Hybridoma 2:253–263
Moll J, Moll R (1985) Cells of extramammary Paget's Disease express cytokeratins different from those of epidermal cells. J Invest Dermatol 84:3–8
Noda Y, Horike H, Watanabe Y, Mori M (1987) Immunohistochemical identification of epithelial membrane antigen in sweat gland tumors by the use of a monoclonal antibody. Pathol Res Pract 182:797–804
Penneys NS, Matsuo S (1989) A monoclonal antibody which identifies an epitope in eccrine duct and acrosyringium. Dermatologica 179:21–24
Penneys NS, Nadji M, Morales A (1982) Carcinoembryonic antigen in benign sweat gland tumors. Arch Dermatol 118:225–227
Penneys NS, Nadji M, Ziegels-Weissmann J, Ketabchi M, Morales AR (1982) Carcinoembryonic antigen in sweat gland carcinomas. Cancer 50:1608–1611
Real FX, Carvato A, Schuessler MH, Welt S, Oettgen HF (1988) IFN-γ-regulated expression of a differentiation antigen of human cells. J Immunol 140:1571–1576
Schmelz M, Duden R, Cowin P, Franke WW (1986) A constitutive transmembrane glycoprotein of Mr 165000 (desmoglein) in epidermal and non-epidermal desmosomes. II. Immunolocalization and microinjection studies. Eur J Cell Biol 42:184–199
Suzuki Y, Hashimoto K, Kato J, Nishioka K, Eto H, Nishiyama S (1989) A monoclonal antibody, SK H1 reacts with 40 kD sweat gland-associated antigen. J Cutan Pathol 16:66–71
Swanson PE, Cherwitz DL, Neumann MP, Wick MR (1987) Eccrine sweat gland carcinoma: an histologic and immunohistochemical study of 32 cases. J Cutan Pathol 14:65–86
Zuk JA, West KP, Fletcher A (1988) Immunohistochemical staining patterns of sweat glands and their neoplasms using two monoclonal antibodies to keratins. J Cutan Pathol 15:8–17
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klein, C.E., Hainzl, A., Kaufmann, R. et al. Cell surface glycoproteins define distinct states of eccrine gland differentiation. Arch Dermatol Res 283, 357–361 (1991). https://doi.org/10.1007/BF00371815
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00371815